<br />Upper
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<br />EXPLANATION
<br />
<br />",,0 Algae sampling sites-
<br />number refers to site
<br />number in table 1
<br />
<br />20
<br />
<br />40 MILES
<br />
<br />20
<br />
<br />40 KILOMETERS
<br />
<br />Figure 1. Location of the Upper Colorado River Basin study unit and algae sampling sites.
<br />
<br />Periphyton microhabitats in RTH environments
<br />include areas within the stream reach that may support
<br />the taxonomically richest assemblage of organisms.
<br />For fixed sites in the Southern Rocky Mountains Prov-
<br />ince of the VCOL study unit, RTH habitats included
<br />riffle areas in shallow, coarse substrate, high-gradient
<br />streams, whereas RTH habitats in the Colorado
<br />Plateaus Province of the VCOL study unit included
<br />riffle areas in shallow, coarse substrate, low-gradient
<br />streams. Five rocks were selected on the basis of size,
<br />periphyton abundance, texture, and color to represent
<br />
<br />the epilithic (rocks, bedrock, and other hard surfaces)
<br />microhabitat distributed throughout the sampling
<br />reach. Individual rocks were placed in a plastic tub
<br />with a small amount of native stream water and were
<br />scraped clean of periphyton by using a stiff-bristled
<br />handbrush as described in Porter and others (1993).
<br />The RTH compo sited algae-water suspension was then
<br />pipetted into a sample container. The periphyton
<br />sample was preserved with a 3- to 5-percent concen-
<br />tration of formalin solution in the field. The foil-
<br />template method (Porter and others, 1993) was used to
<br />
<br />METHODS OF DATA COLLECTION 3
<br />
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