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<br />removal of larval humpback chub (<20 mm) should have less impact on the wild <br />population than removal of an equal number of 50 mm fish. In addition, growing fish <br />from 50 to 150 mm rather than from 30 to 150 mm will take less time in the hatchery <br />(perhaps a month). Two important considerations are to 1) attempt to keep fish held on <br />station for only a year (or less) in order to logistically accommodate the arrival of the <br />next year's stock (i.e., prevent stacking of year classes at a facility), and 2) minimize the <br />time that fish are held in captivity in order to minimize behavioral changes. <br /> <br />Larval humpback chub can be captured with dipnets in the LCR; however, identification <br />to species can be problematic in the field (Childs et al. 1998). Humpback chub 50 to 70 <br />mm can be easily identified by most field personnel, and can be captured and handled <br />with minimum mortality using seines, minnow-traps, or hoop nets. <br /> <br />Table 1. Age (in years), mean total length (mm), and estimated wild annual survival <br />rates for humpback chub. Data provided by C. Walters. <br /> <br />Age <br /> <br />2 <br /> <br />3 <br /> <br />4 <br /> <br />5 <br /> <br />6 <br /> <br />7 <br /> <br />8 <br /> <br />9 <br /> <br />10 <br /> <br />Mean length <br /> <br />93 134 171 <br /> <br />204 232 <br /> <br />258 280 300 318 <br /> <br />334 <br /> <br />Estimated survival rate <br /> <br />0.35 0.52 0.61 <br /> <br />0.67 0.71 <br /> <br />0.73 0.75 0.77 0.78 <br /> <br />0.79 <br /> <br />Another alternative could be removing 1-year old fish during spring. These fish would <br />be -93 mm, and could be grown larger (e.g., 200 mm), for increased survivorship. <br />However, removal of a sufficient number of fish of this size may be more problematic, <br />and should be expected to depress wild recruitment mor~ than removal of smaller fish. <br /> <br />Other considerations will include the logistics of obtaining fish from a variety of <br />locations, the gear types for collecting a desired length of fish, and the logistics of <br />keeping fish alive from the time of collection to arrival at their captive destination. <br />As discussed in the captive broodstock section, collection of fish should be performed <br />on appropriate temporal and spatial scales in order to maintain the maximum likelihood <br />for retention of genetic integrity. Protocols will need to be established to ensure <br />mortality is kept to levels specified in permits. For instance, holding pens will need to be <br />established at each camp, and methods will need to be established for moving fish <br />safely from capture sites to the holding pens. An alternative to setting up holding pens <br />in the river may be large plastic coolers (e.g., 178 quart capacity Gott coolers) supplied <br />with a power source (small Honda generators) and pumps for supplying fresh river <br />water. Aeration of standing water in coolers during the hot ambient air temperatures of <br />July and August is not advised as mortality would occur. Rather, a constant supply of <br />fresh river water is preferred. Fish will need to be treated according to the most recent <br />protocols to relieve stress. Specifics on amounts and types of approved chemicals <br />used to treat fish and relieve stress need to be included in camp protocols, but this <br /> <br />26 <br />