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<br />2. Consider that captive broodstock should be viewed as a last resort, to be <br />performed when all other available conservation actions have failed. <br />3. Should captive propagation be considered and an adequately sized and funded <br />facility be dedicated to that propagation, consider beginning with the already <br />existing small captive group of LCR fish currently being held at Willow Beach <br />NFH, and consider the aggregation of humpback chub at 30-mile as another <br />potential source. <br />4. Build as large a brood stock as possible (several thousand fish), in order to retain <br />as much genetic variability as possible. <br />5. Build the main broodstock from age-O fish collected under a temporal and <br />random spatial design. <br />6. Consider taking several years (5-10) to collect a sufficient amount of age-O fish. <br />7. Once brood stock fish are captured, a complete genetic analysis must be <br />performed under the directions of a formal brood stock management plan in order <br />to avoid problems with introgression, inbreeding, and reduction of Ne (both within <br />the broodstock and into the wild population upon release of these fish). <br />8. Once broodstock(s) and supportive activities are begun, realize that this will be a <br />long-term commitment. <br />9. Realize that release of captive grown fish into the wild will likely need to be a <br />slow, protracted process (many years), in order to avoid potential problems with <br />negatively effecting the wild Ne. <br /> <br />23 <br />