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Table 1. Substrate Design Recipes <br /> Relative Proportion of Substrate Composition <br /> Limestone Sawdust Woodchips AHalfa Manure <br />Cell l 30% 10% 40% 10% 10% <br />Cell2 30% 0% 50% 10% 10% <br />Cella 30% 25% 25% 10% 10% <br />Cell 0 30% 40% 10% 10% 10% <br />Control 30% 19% 31% 10% 10% <br />Substrate ingredients were weighed out on a scale in five-gallon buckets. The subsequent <br />weights were recorded in pounds. The ingredients were thoroughly homogenized by <br />mixing and tightly compacted into the five cells to approximately three inches below the <br />fill line. Cells were then be saturated to approximately one inch above the substrate with <br />Colorado Gulch water and allowed to incubate for twenty days. The incubation time is <br />important to allow bacteria in each cell to establish a strong population prior to the test <br />period. <br />1.4 Summary of Results <br />The bench test was successfully conducted over a period of eight weeks with the <br />exception of Cell 1 which was delayed by five weeks due to leakage of the cell <br />constn~ction. All five cells exhibited successful removal of Al, Cu, and Zn. As shown in <br />Table 2, final zinc removal (weeks 7 and 8) were >50%. <br />Table 2. Zinc Removal from Colorado Gulch Water (Total Recoverable) <br /> Week <br />Cell 1 2 3 4 5 6 7 8 <br />1 -67% 83% 85% 64% 73% 73% 82% NS <br />2 -33% 58% -36% 9% 23% 33% 50% 77% <br />3 -33% 58% 27% 9% 23% 58% 58% 85% <br />4 -33% 58% 55% 9% 23% 50% 58% 77% <br />5 -100% 58% 0% 9% 23% 42% -33% 92% <br />Notes: <br />Negative values indicate a Zn concentration in bioreactor effluent water is greater <br />than Colorado Gulch influent water. <br />4 <br />