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<br />4 <br /> <br />mortality may eliminate the few remaining adults, thus there is a need to evaluate <br />removal of fish for protection in a hatchery facility. It is probably more desirable to <br />remove gametes or y.o.y. fishes than to remove the few remaining adults, however it <br />will likely be more difficult to capture y.o.y. fishes or gametes. <br /> <br />IV. Study Goals, Objectives, End Product: <br /> <br />1. Develop a refugia plan and secure necessary permits for removing fish from the wild <br />and holding them. <br />i. Development of a refugia plan may include examination of genetic samples <br />to evaluate uniqueness of 30-Mile HBC. Development of a refugia plan <br />should be coordinated with development of a genetics management plan. <br />2. Collect adult or juvenile HBC or gametes from the 30-Mile aggregation. <br />i. Number to be collected will be determined as part of the planning process <br />and genetics analysis. <br />3. Prepare annual progress report and final report. <br />4. End product is a refugia population of 30-Mile HBC. <br /> <br />V. Study area <br />1. 30-Mile and vicinity. <br /> <br />VI. Study Methods/Approach <br />1. A refugia plan will be developed by May 2004 and permits will be secured. <br />2. Adult fish will be collected by trammel net during the May-June 2004 period. <br />Y oung-of-the-year fish will be collected by hoop-net, seine, and minnow trap if <br />available and desired, during the July - October period. If gametes are to be <br />removed, sampling should likely take place during May-June. Fish will be removed <br />by the most appropriate method depending on NPS regulations. Fish may be <br />transported to the Little Colorado River for helicopter transport to a suitable <br />hatchery facility. <br /> <br />VII. Task Description and Schedule <br />1. 2003-2004 Develop plan and secure permits. <br />2. 2004 Collect and remove fish or gametes. <br />3. 2005-2020? Maintain fish. <br /> <br />VIII. FY-2003-2004 Work <br />I. Estimated $25,000 - $50,000 to develop a plan; to evaluate and select a refugia <br />location; and to secure permits for removal of genetic material. <br />2. Estimated $40,000 to secure space at a suitable refugia location. <br />3. Estimated $120,000 to capture and move fish from 30-Mile to a refugia location. <br />4. Estimated $IO,OOO/year to maintain fish, depending on brood-stock management <br />plan and genetics evaluation. <br /> <br />IX. Bndget Summary <br />FY-2003 $ 90,000 <br /> <br />Created on 5/6/2003 5:41 PM <br /> <br />n0902 <br /> <br />