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~, I I •lJ • l • InC• III'IIIIIIII"II'11 Doc Date:12/11/2001 <br />Water & Eaviroamealal Testia¢ Inc. 479 West 50 North. American Fork Utah 84003 (801)756-8397 <br />Acute Whole Effluent Toxicity Report ACr 2 3 1996 <br />Fathead Mtnnow <br />Il DATE: October 21, 1996 <br />• <br />PERMITTEE NAME: Western Fuels-Colorado (New Horizon Mine) NPDS NO: OUTFACE NO: 007 <br />TESL' (AnimallAge): Fathead Minnow 6 days. SAMPLE (Date/Type): 10/10/96 grab. <br />DATEITIME TEST BEGAN: 10/11/96 7:30 p.m. DATE/I'B11E TEST COMPLETED: 10/15/96 8:30 p.m. <br />TEST CONDITIONS <br />Fathead Miunow larvae were exposed to diluted effluent as specified by pcrmil. Survival at the cud of the <br />lest period was measured and stalislicallyeyaluafed for Acute Toxicity. <br />,. <br />'Animal;Age;atTest Start .::- <br />Number of Organisms/Dilution Volume/Replicates <br />Foud : :::: ... <br />Aeration <br />.. <br />Dissolved Ox en:: .:. <br />Yg.... ' <br />Water Replacement <br />Tempemturie: , :.: , ~ ~ . ~ ~:.: ~ : , ~ .. ~ .. <br />:. <br />Photo Period <br />PH::..:.. ,: <br />6:days. .: ; . <br />.. .... . <br />5 orgawsms/200 ml/4 replicates. <br />.13rine Shrimp fed.preyiotis to lest .arid .at 48! hours::. <br />None required. <br />.:. <br />Measuie daily old/new:. -:.: <br />,... <br />Renewed every 24 hours, <br />20 -t~=J degecs C. <br />16 hours ambient light/8 hours dark. <br />,.. .... <br />Measyred initially and: ar 24 hours,;:.'.. ::: <br />Reconsliluted ]ab water L15%n hardness of the <br />Receiving Water). <br />:..... <br />Calamrt Draw tslorical Data ' :.' <br />,. <br />Y::. <br />Control, 6.25°l0, ]2.5%, 25%, 50%, 100%. <br />Dilution Water <br />Receipiitg Water .:,:::. <br />Sample Concentrations <br />SUMMARY <br />Results: .~.:X:~. Pass :.~. Fail <br />There was NO significant effeq on survival. <br />Enclosed aze data sheets and statistical reports. <br />Sincerely <br />I'~"" J <br />Lee Rawlings <br />Water 3 Envirotrmental Testing, Inc. <br />Enclosure <br />• <br />