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~.~'. T. Inc. <br />iii iiiiiiiiiiiiiiii <br />999 <br />Acute Whole Effluent Toxicity <br />Fathead Minnow <br />PERMITTEE NAME: Western Fuels Colorado SIGHT ID: New Horizon Mine <br />TEST (Anima(/AgeJ: Fathead Minnow, 7 days. <br />756-8397 ~l <br />'ePo - ~. ~ <br />DATE: October 2, 1997 <br />OUTFACE NO: 007 <br />SAMPLE (Dote/Type): 9/26/97 Grab. <br />DATE/TIME TEST BEGAN: 9/27/97 6:05 p.m. DATE/I IME TEST COMPLETED: 10/2/97 6:15 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end ofthe test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Orgaoisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br />Samole Conceotrati <br />Fathead Minnow, 7 days. <br />10 organisms/I50 mL/2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + 1 degrees C. <br />16 hours light 8 hours dark. . <br />Measured initially and a[ 24 hours. <br />Reconstituted water LI S% hardness of the receiving <br />water). <br />None was supplied. <br />Control, 6.25, 12.5, 25, 50, 100%. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />~~~~.~u~ <br />' ~ee Rawlings <br />Water & Environmental Testing, lnc. <br />Enclosure <br />