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Enviro -Chem Analytical, Inc. Chronic Toxicity Testing <br />2493 Hwy 6850 Unit 7 OXBOW — July 2013 <br />Grand Junction, CO 81505 2 Species <br />Table Of Contents <br />StudySummary ...................................................... ..............................1 <br />Introduction.............................................................. ..............................2 <br />Methods................................................................... ..............................3 <br />Sample Collection ......................................... ..............................2 <br />Sample Preparation and Usage . ............................... ................3 <br />Test Performance .......................................... ..............................4 <br />TestAcceptability ........................................... ..............................5 <br />Statistical Analyses ........................................ ..............................5 <br />Results...................................................................... ............................... 6 <br />Effluent Chemistry Results .............................. ..............................6 <br />Effluent Toxicity Results for Fathead Minnows . ..............................6 <br />Effluent Toxicity Results for Ceriodaphnia ....... ..............................7 <br />References .................................. ............................... ..........8 <br />..................... <br />Table 1. Arrival Chemistry Values for Final Effluent Samples from Elk Creek <br />Mine, Somerset, Colorado <br />Table 2. Initial Chemistry Ranges for Fathead Minnow and Ceriodaphnia <br />dubia 7 -Day Toxicity Tests Conducted with Elk Creek Mine, <br />Test Dates July 23 -30, 2013 <br />Table 3. Final Chemistry Ranges for Fathead Minnow and Ceriodaphnia <br />dubia Toxicity Tests Conducted with Elk Creek Mine <br />- Test Dates July 23 -30, 2013 <br />Table 4. Percentage Survival and Growth as Mean Dry Weight (mg) for <br />Larval Fathead Minnows After 7 -Day Exposure to Elk Creek Mine <br />- Test Dates July 23 -30, 2013 <br />