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Enviro -Chem Analytical, Inc. <br />2493 Hwy 6$50 Unit 7 <br />Grand Junction, CO 81505 <br />Sample Preparation and Usaae <br />Chronic Toxicity Testing <br />OXBOW — July 2013 <br />2 Species <br />The recommended samples were used to perform the effluent tests. Sample <br />information is contained in the table below. <br />Sample Collection <br />Dates and Times <br />Sample <br />Usage Date <br />Sample <br />Volume <br />07/22/13 0800 <br />Test Initiation and Day 1 <br />Renewal <br />4.0 gallons <br />07/24/13 0800 <br />Days 2 and 3 renewals <br />4.0 gallons <br />07/26/13 0800 <br />Days 4, 5, and 6 Renewals <br />4.0 gallons <br />Chain of Custody forms for the samples are contained in Appendix A. <br />Sample preparation followed test guidelines outlined by the USEPA. <br />Test Performance <br />Effluent concentrations of 100 %, 50 %, 25 %, 12.5 %, and 6.25% were utilized <br />during the tests. Concentrations at less than 100% were obtained by diluting the <br />effluent samples with MHW made in the laboratory. All volume measurements <br />were made using graduated cylinders of minimum size. <br />Test temperature for both species was maintained at 25 +/- 1 C. Initial chemistry <br />measurements of pH, dissolved oxygen, temperature and conductivity were <br />performed for each effluent test concentration. Additionally, alkalinity and <br />hardness were performed for the MHW and 100% effluent sample. <br />Final chemistry measurements included temperature, pH, and dissolved oxygen <br />for each concentration and MHW control sample at the end of each renewal <br />period. <br />Fathead Minnows - <br />Four replicates per exposure concentration were tested with 10 organisms per <br />3 <br />