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Aqua -Tox, Inc. <br />4880 Robb Street, Suite 8 <br />... Wheat Ridge, CO 80033 <br />z f ('303) 621 -4880 www aqua -tox com <br />ACUTE TOXICITY REPORT <br />LIENT NAME: Twentymile Coal Compan SAMPLE 1 D.: 051013 -2 <br />% MORTALITY TEST: Pass /-EST RESULT LC50: >100% <br />SURVIVAL IN 100% DILUTION: 100% <br />4MPLE DATE: 05 -09 -13 TIME: 13:30P CLIENT ID: 2000 -115 <br />RGANISM: Daphnia magna AGE: < 24 hours old <br />-ST START: 05-10 -13 10:15A TEST FINISH: 05 -12 -13 10:15A <br />(PE OF TEST: LC50, 48 hours, static renewal moderately hard fresh synthetic dilution water. <br />RATEL <br />AMMONIA: ND <br />HARDNESS: 8947 mg /L <br />ALKALINITY: 54.05 mg /L <br />pH: 7.98 <br />SURVIVAL DATA <br />DATE (HOURS) CONTROL E3.25 <br />5/10/2012 INITIAL <br />5/11/2012 24 <br />5/12/2012 48 <br />MORTALITY: <br />% SURVIVAL: <br />D.0, 7.38 mg /L <br />CHLORINE: ND <br />TEMPERATURE: 20.6 deg C <br />DILUTIONS ( %) <br />12.5 25 t�n 7F Inn <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />100% <br />100% <br />100/ <br />100% <br />100% <br />100% <br />100% <br />- (MIN /MAX) DILUTIONS ( %) <br />0 6.25 125 25 Fn 7ti inn <br />CONDUCTIVITY (micros; <br />D O (mg /L; <br />PH <br />TEMPERATURE (deg C; <br />325/342 <br />602/615 <br />889/906 <br />1386/1423 <br />2360/2400 <br />3130/3270 <br />4040/4110 <br />7.0/74 <br />7.0/7.5 <br />7,0/7.6 <br />1 <br />7.0/7.9 <br />7.0/8.0 <br />7.0/8.1 <br />8,0/8,2 <br />8.3/8.4 <br />8.3/8.5 <br />83/88 <br />83/8.8 <br />8,3/8.7 <br />8.1/8.6 <br />20.0/20.9 <br />20.0/20.9 <br />20,0/20.9 <br />20.0120.9 <br />20.0/20.9 <br />20.0/20.9 <br />20.0/20.9 <br />IEMISTRY - SAMPLE <br />AMMONIA: 0 74 mg /L D O. 8.16 mg /L <br />HARDNESS: 398.19 mg /L CHLORINE: ND <br />ALKALINITY: 880 11 mg /L TEMPERATURE 20.6 deg C <br />pH: 8.14 D.O AFTER AERATION NA <br />ANOEC CALCULATION METHOD METHOD REFERENCE <br />100% SPEARMAN- KARBER EPA - 821 -R -02 -012 October 2002 pg.78 <br />ITIONAL COMMENTS: <br />Analysis ! -� <br />Conducted by: % C' Reviewed by: <br />Date: Date: <br />