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Twentymile Coal Company - ite 1SP Chronic Whole Effluent Toxicity;,,Results <br />2nd Quarter 2013 ATI Lot # 061113 -2abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day static renewal <br />7 -day static renewal <br />Temperature (deg C) <br />25 +/ - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft -c <br />50 -100 ft -c <br />Photoperiod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per cone. <br />10 <br />4 <br />Feeding regime <br />0 2 mL algae/YTC pei day <br />0 15 mL A nauplli 2xdaily <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.48 <br />7.40 <br />7.48 <br />7.96 <br />Dissolved Oxygen (mg /L) <br />6.54 <br />6.25 <br />5.98 <br />7.09 <br />Conductivity (pmhos /cm) <br />1924 <br />1853 <br />1906 <br />328 <br />Ammonia (mg /L) <br />0.06 <br />0.06 <br />0.05 <br />ND <br />Alkalinity (mg /L) <br />532.79 <br />56784 <br />558.83 <br />52.68 <br />Hardness (mg /L) <br />897.80 <br />917.84 <br />934.87 <br />88.98 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />6' >100% <br />Ceriodaphnia dubia <br />Reproduction <br />36.45% <br />25% <br />1 50% <br />Pnnephales promelas <br />Survival <br />>100% <br />100% <br />>100% <br />Plrnephalespromelas <br />Growth <br />02 >100% <br />100% <br />>100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />35.0 <br />12.5% <br />10 <br />100% <br />34.0 <br />25% <br />10 <br />100%: <br />31.7 <br />50% <br />10 <br />100% <br />19.8 <br />75% <br />1,0 <br />100%, <br />10.1 <br />100% <br />10 <br />90% <br />13.3 <br />