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- - o6 <br />Twentymile Coal Company Site 5D Chronic Whole Effluent Toxicity Results <br />2nd (quarter 2013 ATI Lot # 061113 -1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day static renewal <br />7-day static renewal <br />Temperature (deg C) <br />25 +/ - 1 deg C <br />25 +/ - 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft -c <br />50 -100 ft -c <br />Photoperiod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per cone. <br />10 <br />4 <br />Feeding regime <br />0.2mLalyae/YTC per day <br />0,15mLA naup/n2xdady <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />7.71 <br />7.80 <br />8.09 <br />7.96 <br />Dissolved Oxygen (mg /L) <br />7.65 <br />6.80 <br />6.31 <br />7.09 <br />Conductivity (umhos /cm) <br />1431 <br />1399 <br />1412 <br />328 <br />Ammonia (mq /L) <br />0.07 <br />0.06 <br />0.05 <br />ND <br />Alkalinity (mg /L) <br />749.11 <br />764.13 <br />766.14 <br />52.68 <br />Hardness (mq /L) <br />54.71 <br />56.11 <br />53.51 <br />88.98 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction <br />In >100% <br />100% <br />>100% <br />m <br />P�ephales promelas <br />Survival <br />7 >100% <br />100% <br />>100% <br />Pimephales promelas <br />Growth <br />100% <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />33.1 <br />12.5% <br />10 <br />100% <br />35.7 <br />25% <br />10 <br />90% <br />33.4 <br />50% <br />10 <br />100% <br />35.3 <br />75% <br />10 <br />100% <br />34.9 <br />100% <br />10 <br />100% <br />30.1 <br />