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5/30/2013 INITIAL <br />5/31/2013 24 <br />6/1/2013 48 <br />MORTALITY- <br />% SURVIVAL. <br />Aqua -Tax, Inc. <br />20 <br />20 <br />1980 Robb Street, Suite 8 <br />20 <br />„ <br />Wheat Ridge, CO 8003:3 <br />20 <br />(303) 621 -4880 wwvd.aqua -t0X c:om <br />20 <br />20 <br />20 <br />20 <br />20 <br />ACUTE TOXICITY REPORT <br />19 <br />�1ii31Yl.F �f_3 -* <br />Daphnia magna <br />20 <br />CLIENT NAME: Twentymile Coal Company <br />SAMPLE I.D. 053013 -1 <br />0 1 <br />50 % MORTALITY TEST: Pass <br />TEST RESULT LC50: >100% <br />0 <br />% SURVIVAL IN 100% DILUTION: 100% <br />0 <br />0 <br />SAMPLE DATE: 05 -29 -13 TIME: 07:30A <br />CLIENT I . 2000 -84 QC�� <br />100% <br />ORGANISM: Daphnia magna AGE: < 24 hours old <br />100% <br />TEST START: 05 -30 -13 12:50 <br />_ <br />TEST FINISH 06 -01 -13 12:50 <br />TYPE OF TEST- LC50, 48 hours, static renewal moderately hard fresh synthetic dilution water. <br />MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT <br />AMMONIA: ND <br />D. 0.. 7.06 mg /L <br />HARDNESS: 91.68 mg /L <br />CHLORINE: ND <br />ALKALINITY- 53.65 mg /L <br />TEMPERATURE: 20.8 deg C <br />pH: 8.04 <br />SURVIVAL DATA <br />DILUTIONS ( %) <br />DATE HOURS CONTROL 6.25 <br />12,5 25 50 75 100 <br />5/30/2013 INITIAL <br />5/31/2013 24 <br />6/1/2013 48 <br />MORTALITY- <br />% SURVIVAL. <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />19 <br />20 <br />20 <br />20 <br />_ <br />20 <br />20 <br />0 1 <br />1 <br />1 0 <br />0 <br />1 0 <br />0 <br />0 <br />100% 1 <br />95% <br />100% <br />1 100% <br />100% <br />100% <br />100% <br />I <br />DAILY CHEMISTRY - (MIN /MAX) DILUTIONS ( %) <br />0 6.25 12.5 25 50 75 100 <br />CONDUCTIVITY(inicroS) <br />D O (mg /L) <br />pH <br />TEMPERATURE (deg C) <br />3301345 <br />540/606 <br />831 /871 <br />140611452 <br />2360/2490 <br />3380/3480 <br />4370/4480 <br />7.1/7.5 <br />7.2/7.5 <br />7,2/7,6 <br />7.2/7.6 <br />71/7.8 <br />7.3/8.0 <br />7.4/8.4 <br />8.0/8.1 <br />8.1/8.1 <br />8.1/8.3 <br />8.1/8.4 <br />8.1/8.6 <br />8.2/8.7 <br />8.2/8.6 <br />19.9/20.8 <br />19.9/20.8 <br />19.9/20.8 <br />19.9/20.8 <br />19.9/20.8 <br />19.9/20.8 <br />19.9/20.8 <br />GENERAL CHEMISTRY - SAMPLE <br />AMMONIA: 0.05 DO,. 8.38 mg /L <br />HARDNESS: 916.76 mg /L CHLORINE: ND <br />ALKALINITY- 477.66 mg /L TEMPERATURE: 20.8 deg C <br />pH: 8.20 D.0 AFTER AERATION: NA <br />ANOEC CALCULATION METHOD METHOD REFERENCE <br />100% SPEARMAN - KARBER EPA - 821 -R -02 -012 October 2002 pg.78 <br />ADDITIONAL COMMENTS: <br />Analysis 4) � f 44 <br />Conducted by: ((JJ CJ Reviewed by: <br />Date (, Date:! <br />