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Parameter <br />Ceriodaphnia dubia <br />Pimephales promelas <br />Test Type <br />7 -day static renewal <br />7 -day static renewal <br />Temperature (deg C) <br />25 +/ - 1 deg C <br />25 +1- 1 deg C <br />Light Quality <br />ambient lab levels <br />ambient lab levels <br />Light Intensity (footcandles) <br />50 -100 ft-c <br />50 -100 ft -c <br />Photoperiod <br />16 hr light/ 8 hr dark <br />16 hr light/ 8 hr dark <br />Test Chamber Size <br />40 mL <br />500 mL <br />Test Solution Volume <br />30 mL <br />300 mL <br />Renewal of Test Solutions <br />every 24 hours <br />every 24 hours <br />Age of Test Organisms <br />less than 24 hours <br />less than 24 hours <br /># of neonates per test chamber <br />1 <br />10 <br /># replicate test chambers per conc. <br />10 <br />4 <br />Feeding regime <br />0 2 mL algae /YTC per day <br />0,15 mL A nauplu 2xdady <br />Dilution Water <br />MHFSW <br />MHFSW <br />Test Duration <br />7 -8 days <br />7 -days <br />Parameter <br />Sampling Event 1 <br />Sampling Event 2 <br />Sampling Event 3 <br />MHFSW <br />pH <br />8.24 <br />8.23 <br />8.17 <br />8.12 <br />Dissolved Oxygen (mg /L) <br />7,85 <br />7.67 <br />7.42 <br />7 12 <br />Conductivity (pmhos /cm) <br />3630 <br />3560 <br />3550 <br />320 <br />Ammonia (mg /L) <br />ND <br />0.03 <br />0.04 <br />ND <br />Alkalinity (mg /L) <br />350.00 <br />326.00 <br />336.00 <br />55.60 <br />Hardness (mg /L) <br />2094.00 <br />2064.00 <br />2072.00 <br />88.20 <br />Residual Chlorine (mg /L) <br />ND <br />ND <br />ND <br />ND <br />Concentration <br />Number Exposed <br />Percent Survival <br />Mean Average Number of Neonates <br />Control <br />10 <br />100% <br />33.5 <br />12.5% <br />10 <br />100% <br />33.8 <br />25% <br />10 <br />100% <br />313 <br />50% <br />10 <br />100% <br />32.5 <br />75% <br />10 <br />100% <br />17.4 <br />100% <br />10 <br />90% <br />11 9 <br />Species <br />Parameter <br />IC25 <br />NOEC <br />LOEC <br />Ceriodaphnia dubia <br />Survival <br />S. <br />>100% <br />100% <br />>100% <br />Ceriodaphnia dubia <br />Reproduction f, <br />61.94% <br />50% <br />(L. <br />75% <br />Pimephales promelas <br />Survival <br />>100% <br />100% <br />(r <br />>100% <br />Prmephalesprotnelas <br />Growth ( <br />>100% <br />100% <br />>100% <br />Twentymile Coal Company - Site 62 Chronic Whole Effluent Toxicity Results <br />2nd Quarter 2012 ATI Lot # 052212 -1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Ceriodaphnia dubia Survival and Reproduction Data <br />