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ENSR Conaukinp and Fnwi+«ring <br />• A detailed description of the effluent including its source, time of collection, <br />composition, known physical and chemical properties, and any information that <br />appears on the sample container or has been provided by the Sponsor. <br />• The source and characterization of the dilution water, and a description of any <br />pretreatment. <br />• A description of any aeration performed on test solutions before or during the <br />test. <br />• Percentage of test organisms that died in all treatments. <br />• The NOEC (No Observable Effect Concentration) and IC (25 percent reduction <br />in organism performance) for survival and reproduction. <br />• The minimum dissolved oxygen concentration, range in test temperature and <br />pH, and all visual observations of test solutions. <br />• Any deviations from protocol. <br />• Copies of chain of custody records. <br />6.0 LITERATURE CITED <br />Colorado Department of Health. 1993. Guidelines for Conducting Whole Effluent Toxicity <br />Tests. Water Quality Control Division. Denver, Colorado. June 1993. <br />USEPA. 1994. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and <br />Receiving Water to Freshwater Organisms. Third Edition. EPA/600/4-91/002. <br />7.0 PROCEDURAL COMPLIANCE <br />ENSR Project No.: 8503 -096 -193 <br />Protocol No.: C04C0.193.001 <br />Effective: 08/96 <br />Page: 5 of 5 <br />All test procedures, documentation, records, and reports will comply with USEPA (1 994) <br />general guidance on quality assurance related to effluent toxicity testing. To this end, random <br />audits of the test may be scheduled while the test is in progress. The raw data will be <br />checked and compared to protocol requirements and Standard Operating Procedures, and the <br />final report will be audited for accuracy and signed, if satisfactory, by the Study Director and <br />an individual from the Quality Assurance Unit. <br />9.0 PROTOCOL AMENDMENTS AND DEVIATIONS <br />All changes (i.e., amendments, deviations, and final report revisions) of the approved protocol <br />plus the reasons for the changes must be documented in writing. The changes will be signed <br />and dated by the Study Director and maintained with the protocol. <br />9.0 SPONSOR AND STUDY DIRECTOR APPROVAL <br />Sponsor Approval: t A,6.4 _ : r1' 4(C. keit_ ce Date: <br />Study Director: ..n W ► Cr Date: 9/ y/, 4 <br />/ft <br />