2012-06-28_PERMIT FILE - M2012032 (18)

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ENSR Consuhirq and Enpin min; 3.3 Test Containers Test containers will be 30 -m1 plastic beakers containing 15 ml of test solution. 3.4 Photoperiod The photoperiod will be 16 -hours light and 8 -hours dark. 3.5 Dissolved Oxygen Concentrations Dissolved oxygen concentrations will be maintained at z4.0 mg /L. If the dissolved oxygen concentration in the effluent and /or dilution water is low, the effluent and /or dilution water will be aerated prior to preparing the test solutions. 4.1 Test Concentrations 4.2 Number of Test Organisms 4.3 Test Initiation /Renewal Frequency 4.0 TEST DESIGN ENSR Project No.: 8503 -096 -193 Protocol No.: CD4CO.193.001 Effective: 08/96 Page: 3 of 5 WA: COX A vA suit. io /T 7 6 LS* 3S' /6 7S The test concentrations will be 0 (dilution water control), B.237 1.21 2 ,riff', and 100 percent effluent (V:V, effluent:dilution water). A moderately hard reconstituted water (USEPA 1994) control will be conducted concurrently. One Ceriodaphnia dubia will be impartially assigned to each test chamber and ten replicates will be tested per treatment. Testing will be initiated within 36 hours of sample collection. Test chambers will be renewed on a daily basis with freshly prepared dilutions of the most recently received effluent sample. 4.4 Chemical and Physical Monitoring At a minimum, the following measurements will be made: 1. Dissolved oxygen and pH will be measured in each treatment at the beginning and end of each 24 -hour exposure period. 2. Temperature will be measured in test solutions at the beginning of each 24 -hour exposure period and continuously in the environmental chamber or water bath. 3. • ionductivity will be measured in each treatment at the beginning of each 24 -hour exposure period. 4. Hardness, alkalinity, total ammonia, total residual chlorine, pH, and conductivity will be measured in the effluent and the dilution water on the day of sample receipt.