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Test Procedures <br /> Upon receipt at the lab, the water sample was analyzed for ammonia, <br /> alkalinity, hardness, conductivity, dissolved oxygen, and pH. Then the Sodium <br /> Bromide was added to the effluent and the readings were performed again. <br /> Ammonia was determined according to procedures contained in <br /> APHA/AWWA/WPCF (1985). Alkalinity and hardness were determined according to <br /> procedures described in Hach Chemical Company (1978). Conductivity, dissolved <br /> oxygen, and pH meters and probes were used to take these measurements. <br /> The tests followed the procedures in Weber et al. (1989). Sodium Bromide <br /> was added to the AT-004 effluent to produce Bromide concentrations of 1000, 100, <br /> 10, 1, and 0.1 ppm. <br /> Individual Ceriodaphnia were placed in 30 ml plastic containers containing <br /> approximately 15 ml of exposure medium. Ten replicates at each concentration <br /> were used.- The animals were fed daily with an aqueous mixture of trout <br /> chow/alfalfa/yeast and an equal volume of algae (Seienastrum capricornutum). <br /> Exposure medium was changed daily in each container and the number of young <br /> hatching overnight recorded. Young were removed from the containers daily and <br /> discarded. Routine measurements were made each day of temperature, dissolved <br /> oxygen, and pH. <br /> Fathead minnows were exposed in 260 ml plastic cups to which 200 ml of <br /> media was added daily. Ten fish were placed in each jar and four replicates at <br /> each concentration were used. Fish were monitored for survival daily and fed live <br /> Artemia sp. at least twice daily. After seven days, the fish were removed from the <br /> jars and frozen. The fish were weighed on a four place analytical balance after <br /> drying in an oven for two hours at 105°C. <br /> Data Analysis <br /> Data from the test were analyzed on an IBM compatible personal computer <br /> using Fisher's Exact Test, Dunnett's Test, ANOVA with Bonferroni's T-Test, Steel's <br /> Many One Rank, or Wilcoxon's Test. These analyses were performed on the basis <br /> of the normality and heterogeneity of the data. The computer software was part of <br /> the TOXSTAT package developed at the University of Wyoming. <br /> RESULTS <br /> Ceriodaphnia Test Results <br /> Test results for Ceriodaphnia are summarized in Table 1 and provided on the <br /> lab data sheets in Appendix 2. A significant mortality was measured only in the 0.1 <br /> ppm Bromide concentration where 50% of the animals died. This would be the <br /> concentration most like the 100% effluent of the regular quarterly chronic test <br /> (where there was a 60% mortality). Survival in the remaining concentrations ranged <br /> from 70-90%. There was 80% survival in the control. This Control survival <br /> prevented any of the test concentrations from having a statistically significant effect <br />