_GENERAL DOCUMENTS - C1981017 (288)

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C.dubia-MidContinent-Effluent-6/25/96 Program run on 07-01-1996 at 11:19:34 RESULTS CALCULATED USING THE BINOMINAL METHOD -------------------------------------------------------------------------------- CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (PERCENT) -------------------------------------------------------------------------------- 100 20 10 50.00 58.8098 75 20 0 0.00 LESS THAN 0.001 50 20 1 5.00 0.0020 25 20 2 10.00 0.0201 12.5 20 2 10.00 0.0201 AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST SHOWS THAT THE LC50 IS ABOVE 75 THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP HAS NOT BEEN DEMONSTRATED OVER A REASONABLE RANGE (e.g. <37 TO>63 OF PERCENT DEAD 99.99999 CAN BE USED AS AN APPROXIMATE LC50 FOR THIS DATA SET THE MOVING AVERAGE METHOD CANNOT BE USED WITH THIS SET OF DATA BECAUSE NO SPAN PRODUCES MOVING AVERAGES WHICH BRACKET 50 PERCENT DEAD AND ALSO USES TWO CONCENTRATIONS WHICH HAVE PERCENT DEAD BETWEEN 0 AND 100 PERCENT. RESULTS CALCULATED USING THE PROBIT METHOD -------------------------------------------------------------------------------- GOODNESS OF FIT ITERATIONS G H CHI-SQUARE PROBABILITY -------------------------------------------------------------------------------- 5 13.211 4.989 14.967 0.002 BECAUSE THE PROBABILITY IS LESS THAN 0.05, RESULTS CALCULATED USING THE PROBIT METHOD PROBABLY SHOULD NOT BE USED. SLOPE = 1.043831 95 PERCENT CONFIDENCE LIMITS = -2.750194 AND 4.837855 LC50 = 459.7332 95 PERCENT CONFIDENCE LIMITS = 0 AND +INFINITY LC1 = 2.715736 95 PERCENT CONFIDENCE LIMITS = 0 AND 38.68279 COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE REASONABLE. COMMERCIAL TESTING & ENGINEERING CO. 6 Original Original Watermarked For Your Protection