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Q1WOI�If�KKMfff�Hljlll[RJ <br /> Thomas A. Colbert Page 6 <br /> Treatments and Seed Mixture <br /> Each treatment,including the control,was applied to one of the four plots on each <br /> growth medium. All plots were seeded with the same seed mixture. Below are <br /> descriptions of each treatment and of the seed mixture. <br /> TREATMENT 1—CON ROL. The control plots received no amendments. Each <br /> control plot was tilled,raked and seeded only. <br /> TREATMENT 2—ORGANIC AIviENDmwr. To each plot receiving this treatment,a <br /> 1:1 mixture of steer manure and sphagnum peat moss was applied at a rate of 10 <br /> ton/acre (field weight basis). The purpose of this treatment is to enhance the <br /> physical characteristics of the growth media,and to provide nutrients for plants and <br /> an organic matter substrate for soil micro-organisms. <br /> TREATMENT 3--CONTROL OF PH. Treatment 3 consists of a combination of lime <br /> and a proprietary acidifying bacteria inhibitor manufactured by ProMac Systems, a <br /> subsidiary of B. F.Goodrich. This treatment was formulated by ProMac based on <br /> their sampling and analyses of lake sediment and waste rock materials from the site. <br /> Lake sediment plots(I and II)were limed with 1.5 ton/acre calcium carbonate <br /> equivalent; waste rock plots(III)received ProMac only and no lime. ProMac <br /> pellets and powder were applied to each plot in accordance with ProMac's <br /> specifications. Materials were broadcast evenly over each plot area at the following <br /> rates— <br /> ProMac 2000SB — 198 parts <br /> ProMac 2000PN — 84 parts <br /> ProMac 2000PB — 119 parts <br /> ProMac 2000PY — 99 parts <br /> Each of these ProMac materials is formulated to provide different controlled rates of <br /> release of bactericidal active ingredient. The active ingredient is selective for free <br /> living acid-forming autotrophic bacteria(e.gjh obacillus and Ferrobacillus),but <br /> does not affect beneficial heterotrophic bacteria or other types of micro-organisms <br /> in the soil. Results with ProMac technology on acid coal spoil in the eastern United <br /> States have shown great promise for effective long term control of acidification. <br /> i <br /> i <br />