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Evidence also does not seem to support the fact that more easily established shrubs, such as <br />fourwing saltbush, enhance establishment of other shrubs such as big sagebrush; however, <br />inclusion of multiple shrub species in the seed mixture has been shown to increase total shrub <br />seedling density and greater plant community diversity. <br />Research has repeatedly highlighted the many benefits of direct - placed topsoil, such as <br />AM inoculum, better soil physical characteristics, seedbank of native species, healthy microbial <br />populations that ensure good nutrient cycling, and enhanced water infiltration and water storage <br />capacity. Enhanced drought stress tolerance of big sagebrush seedlings when AM associations are <br />present highlights an important factor in improving seedling survival in an arid/semiarid <br />environment where soil moisture levels fluctuate dramatically in the surface few centimeters of the <br />soil. <br />Mulch has also been shown to be critical to formation of "safe sites" for big sagebrush <br />germination and establishment through microclimate modification. Stubble mulching is a desired <br />practice over the use of straw mulch which is more costly, more labor intensive and has a greater <br />potential to introduce non - desired and noxious weed species into reclaimed lands. Use of a stubble <br />mulch has also been shown to have long -term benefits for water infiltration into the reconstructed <br />soil profile (Schuman et al. 1980). <br />Reclamationists have recommended and in some instances planted big sagebrush in small <br />islands with the intention that these islands serve as seed banks for further spread of the species <br />into the revegetated areas. However, data by Gores (1995) and Lyford (1995) showed that natural <br />recruitment of big sagebrush into revegetated mine lands from native stands of big sagebrush was <br />generally limited to a few meters after 10 -15 years. Lyford (1995) stated that natural recruitment <br />decreased 50 -fold when distance to the seed source exceeded 100 m. Therefore, this approach to <br />aiding establishment of big sagebrush will probably not be effective within the bonding time frame. <br />Research within the last decade has produced a much better understanding of seedbed <br />ecology of big sagebrush. Research is leading toward development of a seeding strategy for big <br />sagebrush that should also benefit establishment of other native shrub species as well. <br />References <br />Allen, E.B. 1984. Vesicular - arbuscular mycorrhizae and colonizing annuals: Implications <br />for growth, competition and succession. p. 41 -51. In: S.E. Williams and M.F. Allen (eds). <br />VA Mycorrhizae and Reclamation of Arid and Semiarid Lands. August 17 -19, 1982, <br />Dubois, WY. Wyoming Agr. Exp. Stn. Rpt. No. SAl261, Laramie. <br />Beetle, A.A. and K.L. Johnson. 1982. Sagebrush in Wyoming. Wyoming Agr. Exp. Stn. <br />Bull. 779. University of Wyoming, Laramie. <br />Booth, D.T. 1985. The role of fourwing saltbush in mined land reclamation: A viewpoint. <br />J. Range Manage. 38:562 -565 <br />Booth, D.T., Y. Bai, and E.E. Roos. 1997. Preparing sagebrush seed for market: Effects of <br />debearder processing. J. Range Manage. 50:562 -565. <br />Booth, D.T., J.K. Gores, G.E. Schuman, and R.A. Olson. 1999. Shrub densities on pre -1985 <br />reclaimed mine lands in Wyoming. J. Restoration Ecology. 7:1 -9. <br />Call, C.A. and C.M. McKell. 1982. Vesicular - arbuscular mycorrhizae -a natural revegetation <br />