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% <br />�sz �. <br />-'� <br />-- <br />, <br />f <br />:,303) <br />Aqua -Tox, Inc. <br />4880 Robb Street, Suite 8 <br />Wheat Ridge CO 80033 <br />621 4880 www aqua tox com <br />ACUTE TOXICITY REPORT <br />` - <br />Ceriodaphnia dubia <br />-- ;mil <br />CLIENT NAME: Twentymile <br />100% <br />SAMPLE I.D.. <br />TEST RESULT <br />CLIENT ID. <br />TEST FINISH <br />hard fresh <br />101111 -4 <br />50 % MORTALITY TEST <br />% SURVIVAL IN 100% <br />SAMPLE DATE 10 -10 -11 <br />Pass <br />LC50. <br />2000 -84 <br />>100% <br />DILUTION <br />13.45 <br />TIME 14.30P <br />ORGANISM. Cenodaphnia dubia AGE < 24 hours old <br />10 -13 -11 <br />TEST START. 10 -11 -11 13.45 <br />renewal, moderately <br />TYPE OF TEST LC50, 48 hours, static, <br />synthetic dilution water. <br />MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT <br />AMMONIA <br />HARDNESS <br />ALKALINITY <br />pH <br />ND <br />D O. <br />CHLORINE <br />TEMPERATURE <br />7.02 mg /L <br />86 49 mg /L <br />ND <br />59 86 mg /L <br />20 2 deg C <br />8 12 <br />SURVIVAL DATA <br />DILUTIONS ( %) <br />CONTROL 6 25 12 5 25 50 75 100 <br />DATE (HOURS) <br />10/11/2011 INITIAL <br />10/12/2011 24 <br />10/13/2011 48 <br />MORTALITY <br />% SURVIVAL <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />100% <br />100% <br />100% <br />100% <br />100% <br />100% <br />100% <br />DAILY CHEMISTRY - (MIN <br />/MAX) DILUTIONS ( %) <br />0 6.25 12 5 25 50 75 100 <br />CONDUCTIVITY (microS) <br />D o (mg /L) <br />pH <br />TEMPERATURE (deg C) <br />309/325 <br />590/603 <br />913/928 <br />1445/1469 <br />2680/2760 <br />3750/3870 <br />4750/4780 <br />7 0/7 5 <br />7 2/7 5 <br />7 3/7 5 <br />7 4/7 5 <br />7 4/8 0 <br />7.4/8 5 <br />7 4/9.5 <br />8 0/8 2 <br />8 0/8 3 <br />8 0/8 5 <br />8 1/8 7 <br />8 3/8 8 <br />8.4/8 9 <br />8 6/8.9 <br />20 0/20.2 <br />20.0/20 2 <br />20 0/20 2 <br />20 0/20 2 <br />20 0/20 2 <br />20 0/20 2 <br />20.0/20.2 <br />GENERAL CHEMISTRY <br />- SAMPLE <br />AMMONIA <br />pH <br />ND <br />D.O <br />CHLORINE <br />TEMPERATURE: <br />D 0 AFTER AERATION: <br />10 55 mg /L <br />HARDNESS <br />ALKALINITY <br />838 84 mg /L <br />ND <br />227 96 mg /L <br />20 2 deg C <br />8 94 <br />NA <br />ANOEC <br />CALCULATION METHOD <br />METHOD REFERENCE <br />100% <br />SPEARMAN - KARBER <br />EPA - 821 -R -02 -012 October 2002 pg 78 <br />ADDITIONAL COMMENTS <br />Analysis <br />Conducted by <br />Date <br />1 N 9� <br />Reviewed by <br />Date <br />/ / <br />/ n` /, . <br />6 ° 7 t -- 1 7 I t <br />,= j / /i''' <br />'> f r /0//7-iii <br />