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Sample <br />Time of <br />Collection <br />Date of <br />Collection <br />Time of Receipt <br />Date of Receipt <br />Effluent 1 <br />1030 <br />10 -03 -2011 <br />1000 <br />10 -04 -2011 <br />Effluent 2 <br />1145 <br />10 -05 -2011 <br />0935 <br />10 -06 -2011 <br />Effluent3 <br />1145 <br />10 -07 -2011 <br />0900 <br />10 -08 -2011 <br />Client: Western Fuels <br />Site: New Horizon Mine 007 <br />Test: <br />Client: Western Fuels <br />Test Procedure Followed: <br />Sample Number: <br />SeaCrest Group <br />Dilution Water: <br />Test Organism Source: <br />CO- 0000213 SCG Project No.: 311693.B <br />Chronic Toxicity Test Summary <br />• 7 -day static renewal using Ceriodaphnia dubia. <br />• 7 -day static renewal using Fathead minnow (Pimephales <br />Promelas). <br />• Ceriodaphnia dubia: EPA/821 /R - 02 - 013. Method 1002.0 <br />(2002) <br />• Fathead minnow: EPA/821 /R -02 -013. Method 1000.0 <br />(2002) <br />311693.B <br />• Moderately hard laboratory reconstituted water <br />• Ceriodaphnia dubia <br />SeaCrest Group <br />• Fathead minnow <br />ABS, Inc. <br />Reference Toxicant: • Sodium Chloride /Potassium Chloride <br />3 <br />1 Ceriodaphnia dubia <br />1 Fathead minnow <br />Test Initiation Time <br />1100 <br />1620 <br />Test Initiation Date <br />10 -04 -2011 <br />10 -04 -2011 <br />Test Completion Time <br />1200 <br />1520 <br />Test Completion Date <br />10 -10 -2011 <br />10 -11 -2011 <br />Client: Western Fuels <br />Site: New Horizon Mine 007 <br />Test: <br />Client: Western Fuels <br />Test Procedure Followed: <br />Sample Number: <br />SeaCrest Group <br />Dilution Water: <br />Test Organism Source: <br />CO- 0000213 SCG Project No.: 311693.B <br />Chronic Toxicity Test Summary <br />• 7 -day static renewal using Ceriodaphnia dubia. <br />• 7 -day static renewal using Fathead minnow (Pimephales <br />Promelas). <br />• Ceriodaphnia dubia: EPA/821 /R - 02 - 013. Method 1002.0 <br />(2002) <br />• Fathead minnow: EPA/821 /R -02 -013. Method 1000.0 <br />(2002) <br />311693.B <br />• Moderately hard laboratory reconstituted water <br />• Ceriodaphnia dubia <br />SeaCrest Group <br />• Fathead minnow <br />ABS, Inc. <br />Reference Toxicant: • Sodium Chloride /Potassium Chloride <br />3 <br />