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UUN i F L)L <br />20 <br />U.c.) <br />20 <br />,— <br />20 <br />�., <br />20 <br />- - <br />20 <br />20 <br />20 <br />19 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />19 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />1 <br />100% <br />100% <br />100% <br />100% <br />100% <br />100% <br />95% <br />U <br />345/357 <br />U. /.J <br />663/674 <br />941/948 <br />1518/1550 <br />2510/2530 <br />3520/3590 <br />4500/4610 <br />6.9/7.6 <br />7.1/7.6 <br />7.4/7.7 <br />7.5/7.7 <br />7.5/7.8 <br />7.6/8.0 <br />7.6/8.2 <br />8.1/8.1 <br />8.2/8.3 <br />8.2/8.4 <br />8.2/8.5 <br />8.1/8.6 <br />8.1/8.5 <br />8.1/8.4 <br />19.2/19.9 <br />19.2/19.9 <br />19.2/19.9 <br />19.2/19.9 <br />19.2/19.9 <br />19.2/19.9 <br />19.2/19.9 <br />Aqua -Tox, Inc. <br />4880 Robb Street, Suite 8 <br />Wheat Ridge, CO 80033 <br />(303) 621 -4880 www.aqua- tox.com <br />ACUTE TOXICITY REPORT <br />Daphnia magna <br />CLIENT NAME: Twentymile Coal Company <br />50 % MORTALITY TEST: Pass <br />1 :%, SURVIVAL IN 100% DILUTION: 95% <br />SAMPLE DATE: 07-20 TIME 06:08A <br />ORGANISM. Daphnia »,,.2q,ia AGE: < 24 hours al <br />TEST START: 07 -21 -11 13:45P <br />TEST FINISH: 07 -23 -11 13:45P. <br />TYPE OF TEST: LC50, 48 hours, static, renewal, moderately hard fresh synthetic dilution water. <br />MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT' <br />AMMONIA: ND D.O.: 6.95 mg /L <br />HARDNESS: 97.79 mg /L CHLORINE: ND <br />ALKALINITY: 58.41 mg /L TEMPERATURE: 19.2 deg C <br />pH: 7.99 <br />SURVIVAL DATA <br />DATE too u R ) <br />7/21/2011 INITIAL <br />7/22/2011 24 <br />7/23/2011 48 <br />MORTALITY: <br />SURVIVAL: <br />DAILY CHEMISTRY (MIN /MAX) <br />CONDUCTIVITY (microS) <br />D.O. (mg /L) <br />pH <br />TEMPERATURE (deg C) <br />ANOEC <br />100% <br />ADDITIONAL COMMENTS: <br />Analysis <br />Conducted by <br />Date: < <br />1 <br />z7 <br />d <br />SAMPLE I.D.: 072111 -2 <br />TEST RESULT LC50: >100% <br />CLIENT ID: 2000 -84 <br />DILUTIONS ( %) <br />DILUTIONS NO <br />• O t" <br />GENERAL CHEMISTRY - SAMPLE <br />AMMONIA: 0.09 mg /L <br />HARDNESS: 410.00 mg /L <br />ALKALINITY: 403.44 mg /L <br />pH: 8.05 <br />D.O.: 8.18 mg /L <br />CHLORINE: ND <br />TEMPERATURE: 19.2 deg C <br />D.O AFTER AERATION:. NA <br />CALCULATION METHOD <br />SPEARMAN - KARBER <br />METHOD REFERENCE <br />EPA - 821 -R -02 -012 October 2002 pg.78 <br />Reviewed by: <br />Date: <br />75 <br />75 <br />100 <br />100 <br />