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Larval Fish Growth and Survival Test -7 Day Growth <br />Start Date: 7/19/2011 Test ID: 311468FHM Sample ID: EFF- Effluent <br />End Date: 7/26/2011 Lab ID: SCG- SeaCrest Group Sample Type: EFF2- Industrial <br />Sample Date: Protocol: EPAFW02- EPA/821/R -02 -01 Test Species: PP- Pimephales promelas <br />Comments: <br />Conc -% 1 2 3 4 <br />SN- Control 0.2950 0.1740 0.2889 0.2620 <br />ON- Control 0.2330 0.1740 0.2600 0.2620 <br />20 0.2130 0.2230 0.1880 0.1930 <br />40 0.1930 0.2230 0.1940 0.1940 <br />60 0.1720 0.2500 0.1620 0.1830 <br />80 0.1860 0.2360 0.2200 0.2080 <br />100 0.3030 0.2110 0.1850 0.1890 <br />Transform: Untransformed Isotonic <br />Conc-% Mean N -Mean Mean Min Max CV% N Mean N -Mean <br />SN- Control 0.2550 1.0978 0.2550 0.1740 0.2950 21.905 4 <br />ON- Control 0.2322 1.0000 0.2322 0.1740 0.2620 17.663 4 0.2322 1.0000 <br />20 0.2042 0.8794 0.2042 0.1880 0.2230 8.088 4 0.2063 0.8883 <br />40 0.2010 0.8654 0.2010 0.1930 0.2230 7.301 4 0.2063 0.8883 <br />60 0.1918 0.8256 0.1918 0.1620 0.2500 20.740 4 0.2063 0.8883 <br />80 0.2125 0.9150 0.2125 0.1860 0.2360 9.912 4 0.2063 0.8883 <br />100 0.2220 0.9559 0.2220 0.1850 0.3030 24.863 4 0.2063 0.8883 <br />Auxiliary Tests Statistic Critical <br />Shapiro- Wilk's Test indicates normal distribution (p > 0.05) 0.95655 0.916 <br />Bartlett's Test indicates equal variances (p = 0.21) 7.12683 15.0863 <br />The control means are not significantly different (p = 0.54) 0.65577 2.44691 <br />Linear Interpolation (200 Resamples) <br />Point % SD 95% CL(Exp) Skew <br />ICO5* 8.950 <br />IC10* 17.900 <br />IC15 >100 <br />IC20 >100 <br />1C25. >100 <br />IC40 >100 <br />IC50 >100 <br />* indicates IC estimate Tess than the lowest concentration <br />Page 1 <br />1.0 <br />0.9 - <br />0.8 - <br />0.7 - <br />y 0.6 - <br />c <br />S <br />N <br />1 2 0.4 - <br />0.3 - <br />0.2 <br />0.1 - <br />0.0 <br />0 <br />50 100 150 <br />Dose % <br />ToxCalc v5.0.23 Reviewed by: <br />Skew Kurt <br />0.75496 1.16571 <br />