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20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />_ <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />20 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />0 <br />100% <br />100% <br />100% <br />100% <br />100% <br />100% <br />100% <br />333/345 <br />844/862 <br />1368/1379 <br />1874/ 888 <br />2370/2390 <br />3390/3420 <br />4410/4440 <br />7.0/7.3 <br />7.0/7.03 <br />7.1/7.4 <br />7.1/7.5 <br />7.2/7.5 <br />7.3/7.5 <br />7.4/7.6 <br />8.0/8.4 <br />7.9/8'4 <br />7.8/8.5 <br />78/8.6 <br />7.7/8.7 <br />7.7/8.8 <br />7.6/89 <br />19.2/19.0 <br />19.2/19.9 <br />19.2d9.9 <br />19.280.9 <br />19.2/19.9 <br />19.2d9.0 <br />18.2U0.9 <br />CLIENT NAME: TwentymUo Coal Company <br />5D96 MORTALITY TEST: Pass <br />% SURVIVAL IN 100% DILUTION: 10096 <br />SAMPLE DATE: 05'20'11 TIME: 11:0OA <br />ORGANISM: Ceriodaphnia dubia AGE: <24 hours old <br />TEST START: 05`27-11 TEST FINISH: 05-29-11 <br />TYPE OF TEST: LC50. 48 houra, ntatic, renawa|, moderately hard fresh synthetic dilution water. <br />MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT <br />AMMONIA: ND D.O.: 6.95 mg/L <br />HARDNESS: 97.79 mg/L CHLORINE: ND <br />ALKALINITY: 58.41 mg/L TEMPERATURE: 19.2 deg C <br />pH: 7.99 <br />SURVIVAL DATA <br />DATE (HOURS) CONTROL <br />5/27/2011 INITIAL <br />5/28/2011 24 <br />5/20/2011 48 <br />MORTALITY: <br />% SURVIVAL: <br />DAILY CHEMISTRY - (MIN/MAX) <br />CONDUCTIVITY (microS) <br />D.D. (mg/L) <br />pH <br />TEMPERATURE (deg C) <br />GENERAL CHEMISTRY - SAMPLE <br />AMMONIA: 0.08 <br />HARDNESS: 2,388.68 mg/L <br />ALKALINITY: 599.63 mg/L <br />pH: 7.37 <br />ADDITIONAL COMMENTS: <br />Analysis <br />Conducted by: <br />Date: <br />Aqua-Tox„ Inc. <br />4880 Robb Street, Suite 8 <br />Wheat Ridge, CO 80033 <br />(303) 621-4880 www.aqua-tox.com <br />ACUTE TOXICITY REPORT <br />Ceriodaphnia dubia <br />8AMPLEiD.: 052711-1 <br />TEST RESULT LC50: >100% <br />CLIENT ID: Site 62 <br />DILUTIONS (°/0) <br />6.25 12.5 25 50 <br />DILUTIONS (%) <br />0 6.25 12.5 25 50 <br />Reviewed by: <br />Date: <br />7 5 <br />D.O.: 7.59 mg/L <br />CHLORINE: ND <br />TEMPERATURE: 19.2 deg C <br />D.0 AFTER AERATION: NA <br />ANOEC <br />� - ' CALCULATION METHOD (. �'� <br />SPEARMAN-KARBER <br />METHOD REFERENCE �� <br />EPA -821-R-02-012 October 2002 pg.78 <br />