2011-05-03_HYDROLOGY - C1982056 ‎(2)‎

• Permit No: C1982056
• IBM Index Class Name: HYDROLOGY
• Doc Date: 5/3/2011
• Doc Name: 1st Quarter 2011 DMRS ‎(CO-0042161)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JDM
• Media Type: D
• Archive: No

Aqua-Tox, Inc.
4880 Robb Street, Suite 8
f > Wheat Ridge, CO 80033
[phone] aqua-tox.com
ACUTE TOXICITY REPORT
Ceriodaphnia dubia
CLIENT NAME: 20-Mile Coal Company SAMPLE I.D.: 011811-1
50 % MORTALITY TEST: Pass TEST RESULT LC50: >100%
% SURVIVAL IN 100% DILUTION: 75%
SAMPLE DATE: 01-17-11 TIME: 10:OOA CLIENT ID: Site 2000-115
ORGANISM: Ceriodaphnia dubia AGE: < 24 hours old
TEST START: 01-18-11 12:20P TEST FINISH: 01-20-11 12:20P
TYPE OF TEST: LC50, 48 hours, static, renewal, moderately hard fresh synthetic dilution water.
MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT
AMMONIA: ND D.O.: 6.92 mg/L
HARDNESS: 97.84 mg/L CHLORINE: ND
ALKALINITY: 57.20 mg/L TEMPERATURE: 19.7 deg C
pH: 8.00
iURVIVAL DATA DILUTIONS (%)
DATE (HOURS)CONTROL 6.25 12.5 25 50 75 100
1/18/2011 INITIAL
1/19/2011 24
1/20/2011 48
MORTALITY:
% SURVIVAL:
20 20 20 20 20 20 20
20 20 20 19 20 19 18
20 20 20 19 20 19 15
0 0 0 1 0 1 5
100% 100% 100% 95% 100% 95% 75%
41LY CHEMISTRY - (MIN/MAX) DILUTIONS (%)
0 6.25 12.5 25 50 75 100
CONDUCTIVITY (microS)
D.O. (mg/L)
pH
TEMPERATURE (deg C)
315/329 961/978 1647/1664 2270/2300 2930/2960 4240/4280 5510/5550
6.9/7.3 7.0/7.4 7.1/7.4 7.2/7.5 7.3/7.5 7.4/7.6 7.5/7.7
8.0/8.4 8.0/8.4 8.0/8.5 8.0/8.5 8.0/8.6 8.0/8.7 8.0/8.8
19.7/20.6 19.7/20.6 19.7/20.6 19.7/20.6 19.7/20.6 19.7/20.6 19.7/20.6
GENERAL CHEMISTRY - SAMPLE
AMMONIA: 0.73 mg/L D.O.: 7.72 mg/L
HARDNESS: 474.19 mg/L CHLORINE: ND
ALKALINITY: 950.00 mg/L TEMPERATURE: 19.7 deg C
pH: 8.01 D.0 AFTER AERATION: NA
NF' ANOEG`X',??„«x?t CALCULATION<METHODY'...`-? . , [Y`NV'1`4'Z.'?,-ZMETHOD -REFERENCE'
75% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg.78
NAL COMMENTS:
Analysis e
Conducted by: Reviewed by:
Date: t 2 Date: ,/7 ?Jj 1