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Client: Copper Mountain CMD CO-0021598 SCG Project No.: 310921 <br />Site: WWTP <br />Table 1. Statistics methods used in testing for significant differences in test parameters. <br />Species Normality Homogeneity <br />Ceriodaphnia dubia Kolmogorov Test Bartlett's Test <br />Fathead minnow Shapiro - Wilk's Test Bartlett's Test <br /> Statistical Difference <br /> Survival Growth Reproduction IC25 <br />Ceriodaphnia dubia N/A N/A Dunnett's Test ICp <br />Fathead minnow Dunnett's Test Dunnett's Test N/A ICp <br />RESULTS <br />Ceriodaphnia dubia Test Results <br />Test results for the Ceriodaphnia dubia are summarized in Table 2 and provided on the <br />data sheets located in Appendix 2. Survival was 100% in the 100% effluent concentration and <br />100% in the remaining effluent concentrations. Control survival was 100%. No statistically <br />significant mortality was measured in any effluent concentration. The IC25 for survival was <br />> 100%. <br />Table 2. Summary of Ceriodaphnia dubia test results. Ten animals were exposed at each <br />concentration. An asterisk (*) denotes a statistically significant difference from the control. <br /> <br />No <br />M Significant Difference <br /> <br />Concentration . <br />Surviving ean <br />Births <br />Min. <br />Max. <br />Survival <br />Re rod. <br />Control (0%) 10 17.9 10 29 <br />12.5% 10 15.8 8 21 <br />25% 10 14.7 10 21 <br />50% 10 13.1 6 21 <br />75% 10 15.1 4 22 <br />100% 10 15.2 11 23 <br />Average numbers of neonates was 15.2 in the 100% effluent concentration and ranged <br />from 14.7 - 15.8 in the remaining effluent concentrations. Average number of neonates in the <br />control was 17.9 for statistical analyses and 17.9 for test acceptability criteria. No statistically <br />significant differences in the number of neonates were found between the control and any <br />effluent concentration. The IC25 for reproduction was > 100%. <br />SeaCrest Group 7