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Client: Cotter Corporation <br />Site: 001A <br />SCG Project No.: 310921 <br />Quality Assurance Check List - Chronic Whole Effluent Toxicity Test <br />Client: Cotter Corporation SeaCrest Sample No.: 310921 <br />Species Tested: Ceriodaphnia dubia and Fathead minnow <br />Start Date of Test Start Date of Test <br />(Ceriodaphnia dubia) (Fathead minnow) <br />09-09-2010 09-16-2010 <br />Sample received in lab properly preserved (0-60C)? N* <br />Sample delivered on ice or equivalent? Y <br />Test initiated within 36-hours of collection? Y <br />Test protocol conforms to CDPHE guidelines (Ceriodaphnia dubia)? Y <br />Test protocol conforms to CDPHE guidelines (Fathead minnow)? Y <br />Average test temp. f 1 °C (Ceriodaphnia dubia)? Y <br />Average test temp. t 1 °C (Fathead minnow)? Y <br />DO level >4.Omg/L; no super-saturation (Ceriodaphnia dubia)? Y <br />DO level >4.Omg/L; no super-saturation (Fathead minnow)? Y <br />Survival in control >90%, >80% for chronic (Ceriodaphnia dubia)? Y <br />Survival in control >90%, >80% for chronic (Fathead minnow)? Y <br />Ceriodaphnia dubia neonates in control <24-hours old? Y <br />Fathead minnow larvae in control 1 to 14 days old? Y <br />Appropriate reference toxicity test conducted? Y <br />Lab. Ref. Tox. test results within the confidence limits for the lab? Y <br />*All samples hand delivered on the same day were received at above temperature. <br />Position: Lab Director <br />as?-1010 <br />SeaCrest Group 29