Laserfiche WebLink
METHOD <br /> A grab sample was collected from the Tenmile Drainage, at Climax <br /> Mine, on December 12 , 1994 , delivered to Commercial Testing and <br /> Engineering (CT&E) on December 13 , 1994, and assigned CT&E number <br /> 72-294415. A two species acute bioassay was initiated <br /> approximately 28 hours following sample collection. <br /> Testing was conducted in accordance with the most recent protocols <br /> specified by the Water Quality Control Division of the Colorado <br /> Department of Health (1993) , outlined in Table 1. <br /> Basic characteristics of the effluent and laboratory dilution water <br /> were measured upon sample arrival at CT&E. The pH was measured <br /> using a Fisher Scientific model 925 pH meter (EPA method 150. 1) and <br /> ammonia was measured with an Orion ionalyzer and an Orion selective <br /> ionic electrode (EPA method 350. 3) . Total residual chlorine was <br /> measured using a LaMotte model STC-Cl colorimeter (EPA accepted) . <br /> Alkalinity and hardness were determined by titrimetric methods <br /> specified by the American Public Health Association, 1989 (EPA <br /> methods 310. 1 and 130. 2) . Dissolved oxygen was measured with a YSI <br /> model 54A oxygen meter (EPA method 360. 1) and conductivity was <br /> measured with a YSI Model 31 conductivity bridge (EPA method <br /> 120. 1) . <br /> The tests exposed cladocerans, Ceriodaphnia dubia and fathead <br /> minnow, Pimephales promelas to five concentrations of effluent, <br /> 100%, 75*, 50%, 25%, and 12 . 5%, prepared using reconstituted <br /> laboratory water at a hardness of 100 mg/L CaCO3 . Control <br /> replicates were exposed to 100% laboratory water. <br /> LC50 values were determined with the Probit Method. <br /> -2 <br /> COMMERCIAL TESTING & ENGINEERING CO. <br /> F-466 <br /> Original Watermarked For Your Protection = <br />