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Aqua-Tox, Inc. <br />4880 Robb Street, Suite 8 <br />Wheat Ridge, CO 80033 <br />(303) 621-4880 aqua-tox.com <br />ACUTE TOXICITY REPORT <br />Ceriodaphnia dubia <br />CLIENT NAME: 20-Mile Coal Companv SAMPLE I.D.: 052610-2 <br />50 % MORTALITY TEST: Pass TEST RESULT LC50: >100% <br />% SURVIVAL IN 100% DILUTION: 60% <br />SAMPLE DATE: 05-25-10 TIME: 09:19A CLIENT ID: Site 87 <br />ORGANISM: Ceriodaphnia dubia AGE: < 24 hours old <br />TEST START: 05-26-10 10:50A TEST FINISH: 05-28-10 10:50A <br />TYPE OF TEST: LC50. 48 hours, static, renewal moderately hard fresh synthetic dilution water. <br />AMMONIA: ND <br />HARDNESS: 92.19 mg/L <br />ALKALINITY: 57.81 mg/L <br />pH: 8.01 <br />iURVIVAL DATA <br />DATE HOURS CONTROL 6.25 <br />5/26/2010 INITIAL <br />5/27/2010 24 <br />5/28/2010 48 <br />MORTALITY: <br />% SURVIVAL: <br />D.O.: 7.16 mg/L <br />CHLORINE: ND <br />TEMPERATURE: 19.7 deg C <br />DILUTIONS (%) <br />17 A Or <br />cn ?. <br /> <br />20 20 <br />20 <br />20 <br />20 <br />20 ivy <br />20 <br />20 20 20 20 20 18 15 <br />20 20 20 20 20 18 12 <br />0 0 0 0 0 2 8 <br />100% 100% 100% 100% 100% 90% 60% <br />ILY CHEMISTRY - (MIN/MAX) DILUTIONS (%) <br />0 A 7.ri 17 F 7r cn .r <br />CONDUCTIVITY (microS. <br />D.O. (mg/L) <br />pH <br />TEMPERATURE (deg C; <br /> <br />311/329 <br />471/488 <br />642/661 <br />1028/1050 <br />1690/1723 <br />2400/2440 ivy <br />2780/2820 <br />7.2/7.4 7.2/7.4 7.2/7.4 7.2/7.4 7.2/7.4 7.2/7.4 7.2/7.5 <br />8.0/8.3 8.0/8.4 8.0/8.5 8.0/8.5 8.0/8.6 8.0/8.7 8.018.8 <br />19.7/19.9 19.7/19.9 19.7/19.9 19.7/19.9 19.7/19.9 19.7/19.9 19.7/19.9 <br />GENERAL CHEMISTRY - SAMPLE <br />AMMONIA: ND D.O.: 7.22 mg/L <br />HARDNESS: 1,157.33 mg/L CHLORINE: ND <br />ALKALINITY: 99.63 mg/L TEMPERATURE: 19.7 deg C <br />pH: 7.95 D.O AFTER AERATION: NA <br />` ANOEC CALCULATION-'METHOD METHOD REFERENCE <br />50% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg.78 <br />ADDITIONAL <br />Analysis <br />Conducted by: ? Reviewed by: <br />Date. Date: