2010-08-01_HYDROLOGY - C1982056 ‎(2)‎

• Permit No: C1982056
• IBM Index Class Name: HYDROLOGY
• Doc Date: 8/1/2010
• Doc Name: 2nd Quarter 2010 DMRS ‎(CO-0027154)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JDM
• Media Type: D
• Archive: No

Aqua-Tox, Inc.
4880 Robb Street, Suite 8
Wheat Ridge, CO 80033
[phone] aqua-tox.com
ACUTE TOXICITY REPORT
Ceriodaphnia dubia
.TENT NAME: 20-Mile Coal Company SAMPLE I.D.: 060810-1
% MORTALITY TEST: Pass TEST RESULT LC50: >100%
SURVIVAL IN 100% DILUTION: 65%
MPLE DATE: 06-07-10 TIME: 12:OOP CLIENT ID: Site 84
;GANISM: Cerioda hnia dubia AGE: ..< 24 hours old
ST START: 06-08-10 11:50A TEST FINISH: 06-10-10 11750A
PE OF TEST: LC50 48 hours static renewal moderate) hard fresh synth tic dilution water.
AMMONIA: ND
HARDNESS: 96.59 mc?lL
ALKALINITY: 57.8_ 1 mg/L
PH: 7.92
D.O.: 7.22 mg/L
CHLORINE: ND
TEMPERATURE: 20.3 deg C

DATE HOURS
CONTROL
6.25
12
5 DILUTIONS (%)
25

6/8/2010 INITIAL
20
20 .
20
20 50

20 75
100
6/9/2010 24 20 20
20
20
20 20
19 20

6/10/2010 48
20
20
20 15
20 20 17
MORTALITY: 0 0 0 0

% SURVIVAL:

100%

100%

1
0
3 7
7
00% 100% 100% 85% 65%

AILY CHEMISTRY - (MIN/MAX) DILUTIONS (%)

CONDUCTIVITY (micros) 0
323/329 6.25
366/373 12.5
4 25 50 75 100
02/408 441/448 485/497 536/554

D.O. (mg/L)
7
2/7
4
7
1/7
4
7 605/626
.
. .
. .1/7.4 7.1/7.3 7.2/7.4 7
3/7
4 7
4/7
5
TEMPERATUREp (deg c) 19.9/20 4 19.9/20.4 19
9/20 4 19
9/20
4 .
. .
.
.97
. .
. 19.9/20.4 19.9/20.4 19.9/20.4
IERAL CHEMISTRY - SAMPLE
AMMONIA: 1.21 mg/L D.O.: 7.44 mg/L
HARDNESS: 505.42 mac /L CHLORINE: D
ALKALINITY: 772.34 mg/L TEMPERATURE: 20.3 deg C
PH: 7.94 D.O AFTER AERATION: NA
- ---------------
ANOEC CALCULATION" METHOD = "METHQD`REFERENCE
50% SPEARMAN-KARBER EPA -821-R-02-012 October 2002
Pg.78
ADDITIONAL COMMENTS-,
Analysis
Conducted by:
Date:
Reviewed by:
Date:
U/:,/,c