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Twentymile Coal Company . Site 1SP Chronic Whole Effluent Toxicity Results <br />2nd Quarter 2010 ATI Lot # 061510-1 abc <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter Ceriodaphnia dubia Pimephales promelas <br />Test Type 7-day static renewal 7-day static renewal <br />Temperature (deg C) 25 +/ - 1 -deg C 25 +/ - 1 deg C <br />Light Quality ambient lab levels ambient lab levels <br />Light Intensity (footcandles) 50-100 ft-c 50-100 ft-c <br />Photoperiod 16 hr light/ 8 hr dark 16 hr light/ 8 hr dark <br />Test Chamber Size 40 mL 500 mL <br />Test Solution Volume 30 mL 300 mL <br />Renewal of Test Solutions every 24 hours every 24 hours <br />Age of Test Organisms less than 24 hours less than 24 hours <br /># of neonates per test chamber 1 10 <br /># replicate test chambers per conc. 10 4 <br />Feeding regime 0.2 ML algaeNTC per day 0.15 mL A. nauplil 2xdaily <br />Dilution Water <br />1 MHFSW MHFSW <br />Test Duration <br />77 7-8 days 7-days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter Sampling Event 1 Sampling Event 2 Sampling Event 3 MHFSW <br />pH 7.63 7.52 7.27 7 <br />95 <br />Dissolved Oxygen (mg/L) 5.20 6.52 5.94 . <br />6 <br />83 <br />Conductivity (pmhos/cm) 2310 2460 1814 . <br />309 <br />Ammonia (mg/L) ND ND ND ND <br />Alkalinity (mg/L) 622.69 575.03 458.69 57.81 <br />Hardness (mg/L) 750.50 950.90 746.49 g6 <br />gg <br />Residual Chlorine (mg/L) ND ND ND , <br />ND <br />Ceriodaphnia dubia & Pimephales promelas Chronic Statistical Endpoints <br />Species Parameter IC25 NOEC LOEC <br />Ceriodaphnia dubia Survival >100% 100% >100% <br />Ceriodaphnia dubia Reproduction >100% 100% >100% <br />Pimephalespromelas Survival >100% 100°/% >100% <br />Pimephalespromelas Growth >100% 100% >100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration Number Exposed Percent Survival Mean Average Number of Neonates <br />Control 10 100% 35.4 <br />12.5% 10 100% 34.7 <br />25% 10 100% 34.8 <br />50% 10 100% 34.8 <br />75% 10 100% 33.3 <br />100% 10 100% 35.6