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Client: Western Fuels CO-0000213 SCG Project No.: 310547 <br />Site: New Horizon Mine NPDES 011 <br />Acute Toxicity Test Summary <br />Test: • 48-hour static renewal using Ceriodaphnia dubia <br />• 96-hour static renewal using Fathead minnow <br />(Pimephales promelas) <br />Client: Western Fuels <br />Test Procedure Followed: Ceriodaphnia dubia: EPA/821/R-02-012. Method 2002.0 <br />(2002) <br />Fathead minnow: EPA/821 /R-02-012. Method 2000.0 <br />(2002) <br />Sample Description: 310547 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Receipt Receipt <br />1130 05-24-2010 1038 05-25-2010 <br /> Cerinda hniu dubia Fathead minnow <br />Test Initiation Time 1350 1330 <br />Test Initiation Date 05-25-2010 05-25-2010 <br />Test Completion Time 1330 1350 <br />Test Completion Date 05-27-2010 05-29-2010 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Ceriodaphnia dubia <br />SeaCrest Group <br />• Fathead minnow <br />SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />SeaCrest Group 3