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Client: Western Fuels CO-0000213 SCG Project No.: 310624 <br />Site: New Horizon Mine 007 <br />DISCUSSION <br />A failed test for this discharge occurs whenever there is a statistically significant <br />difference in lethality between the control and any effluent concentration <_ the 1WC (Instream <br />Waste Concentration) of 100%. Since neither test species had significant mortality meeting this <br />criterion, the discharge passes WET testing requirements. <br />REFERENCES <br />1. Hach Chemical Company. 2008. Hach 's Water Analysis Handbook. Fifth Edition. Hach <br />Chemical Company, Loveland, Colorado. Digital Medium. <br />2. APHA/AWWA/WEF. 1998. Standard Methods for the. Examination of Water and <br />Wastewater. 20th Edition. American Public Health Association, Washington, D.C. <br />3. USEPA. 2002. Short Term Methods for Estimating the Chronic Toxicity of Effluents and <br />Receiving Waters to Freshwater Organisms. EPA-821-R-02-013. 335 pp. <br />4. CDPHE (Colorado Department of Public Health and Environment). 1998. Laboratory <br />Guidelines for Conducting Whole Effluent Toxicity Tests. Water Quality Control Division. <br />5. USEPA. 2000. Method of Guidance and Recommendations for Whole Effluent Toxicity <br />(WET) Testing (40 CFR Part 136). EPA/821/B-00/004. <br />6. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent <br />Toxicity Applications under the National Pollutant Discharge Elimination System Program. <br />EPA/833/R-00/003. <br />SeaCrest Group 9