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Client: Western Fuels CO-0000213 SCG Project No.: 310624 <br />Site: New Horizon Mine 007 <br />Table 1. Statistics methods used in testing for significant differences in test parameters. <br />Species Normality Homogeneity <br />Ceriodaphnia dubia Kolmogorov Test Bartlett's Test <br />Fathead minnow Shapiro - Wilk's Test Bartlett's Test <br /> Statistical Difference <br /> Survival Growth Reproduction IC25 <br />Ceriodaphnia dubia N/A N/A Dunnett's Test ICp <br />Fathead minnow Steel's Many-One Rank Dutuiett's Test N/A ICp <br />RESULTS <br />Ceriodaphnia dubia Test Results <br />Test results for the Ceriodaphnia dubia are summarized in Table 2 and provided on the <br />data sheets located in Appendix 2. Survival was 100% in all effluent concentrations. Control <br />survival was 100%. No statistically significant mortality was measured in any effluent <br />concentration when compared to the control. The IC25 for survival was >100%. <br />Table 2. Summary of Ceriodaphnia dubia test results. Ten animals were exposed at each <br />concentration. An asterisk (*) denotes a statistically significant difference from the control. <br /> Significant Difference <br /> <br />Concentration No. <br />Surviving Mean <br />Births <br />Min.,,-,. <br />Max. <br />Survival <br />Re rod. <br />Control (0%) 10 16.7 5 33 <br />6.25% 10 16.7 13 23 <br />12.5% 10 14.6 9 19 <br />25% 10 14.1 9 24 <br />50% 10 14.4 8 20 <br />100% 10 18.5 11 24 <br />Average numbers of neonates was 18.5 in the 100% effluent concentration and ranged <br />from 14.1 - 16.7 in the remaining effluent concentrations. Average number of neonates in the <br />control was 16.7. No statistically significant differences in the number of neonates were found <br />between the control and any effluent concentration. The IC25 for reproduction was >100%. <br />SeaCrest Group