2010-02-01_HYDROLOGY - C1982056 ‎(4)‎

• Permit No: C1982056
• IBM Index Class Name: HYDROLOGY
• Doc Date: 2/1/2010
• Doc Name: 4th Quarter 2009 December DMRS ‎(CO-0027154)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JHB
• Media Type: D
• Archive: No

Aqua-'Tox, Inc.
4880 Robb Street, Suite 8
Wheat Ridge, CO 80033
[phone] aqua-tox.com
ACUTE TOXICITY REPORT
Cerioda hnia dubia
CLIENT NAME: Twentymile Coal Company SAMPLE I.D.: 101509-7
50 % MORTALITY TEST: Pass TEST RESULT LC50: >100%
% SURVIVAL IN 100% DILUTION: 80%
SAMPLE DATE: 10-14-09 TIME: 15:30PM CLIENT ID: Site 84
ORGANISM: Ceriodaphnia dubia AGE: < 24 hours old
TEST START: 10-15-09 11:45AM TEST FINISH: 10-17-09 11:45AM
TYPE OF TEST: LC50, 48 hours, static, renewal, moderately hard fresh synthetic dilution water.
MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT
AMMONIA: ND D.O.: 7.06 mg/L
HARDNESS: 92.00 mg/L CHLORINE: ND
ALKALINITY: 54.42 mg/L TEMPERATURE: 19.7 deg C
pH: 8.10
SURVIVAL DATA DILUTIONS (%)
DATE (HOURS)CONTROL 6.25 12.5 25 50 75 100
10/15/2009 INITIAL 20 20 20 20 20 20 20
10/16/2009 24 20 20 20 20 20 20 20
10/17/2009 48 20 20 20 20 20 20 16
MORTALITY: 0 0 0 0 0 0 4
% SURVIVAL: 100% 100% 100% 100% 100% 100% 80%
I
DAILY CHEMISTRY - (MIN/MAX) DILUTIONS (%)
0 6.25 12.5 25 50 75 100
CONDUCTIVITY (microS) 314/333 706/715 99911012 1585/1594 2950/3020 4090/4200 5280/5430
D.O. (mg/L) 7.1/7.2 7.1/7.2 7.2/7.2 7.1/7.4 7.1/7.6 7.1/7.9 7.1/8.4
pH 7.9/8.2 8.0/8.2 8.1/8.3 8.3/8.4 8.5/8.6 8.5/8.7 8.4/8.8
TEMPERATURE (deg C) 19.7/20.4 19.7/20.4 19.7/20.4 19.7/20.4 19.7/20.4 19.7/20.4 19.7/20.4
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GENERAL CHEMISTRY - SAMPLE
AMMONIA: ND D.O.: 8.37 mg/L
HARDNESS: 760.00 mg/L CHLORINE: ND
ALKALINITY: 520.72 mg /L TEMPERATURE: 19.7 deg C
pH: 8.44 D.O AFTER AERATION: NA
MOM CALCULATIONMETHOD : METHOD ,REFERENCE
75% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg.78
ADDITIONAL COMMENTS:
Analysis
Conducted by: - , o • z , . 9 Reviewed by:
Date: Date o a, a