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7. II -W mine, fenced site 1, transplanted aspen plants, dozer - cleared /stored <br />topsoil, high irrigation <br />8. II -W mine, fenced site 1, transplanted aspen plants, dozer - cleared /stored <br />topsoil, low irrigation <br />9. II -W mine, fenced site 1, root segment natural aspen sprouts, roto- <br />cleared /fresh topsoil, no irrigation <br />10. II -W mine, fenced site 1, root segment natural aspen sprouts, dozer- <br />cleared /stored topsoil, no irrigation <br />11. II -W mine, fenced site 2, planted potted aspen seedlings, dozer- <br />cleared /stored topsoil, no irrigation <br />12. Yoast mine, non - fenced site, natural aspen sprouts <br />Treatment 12 was studied only in 2005. Most of the aspen plants in this unfenced <br />treatment were severely damaged by ungulates, verifying the importance of fencing for <br />aspen establishment. All data collected in 2006 were from fenced plots and do not <br />• reflect the devastating effect of browsing by wild ungulates without the benefit of <br />fencing. <br />Data Collected: <br />Prior to bud break initial height of each tree and number of branches was <br />recorded for each tree. Tree growth was measured periodically throughout the growing <br />season. Physical measures of growth were total terminal and branch growth (cm), <br />crown height (cm), basal caliper (mm), and number of basal sprouts (count). Water <br />status of the trees was measured twice during the summer of 2006. <br />Water status, determined as leaf water potential of the plants, was measured as <br />near to dawn as possible ('/z hr predawn to' /Z hr after sunup) to capture the minimum <br />stress before rapid morning transpiration began depleting leaf moisture. Treatment, <br />ambient temperature, time of sampling and exuding pressure level was recorded. <br />Leaves were collected from the different treatments at random to minimize time of <br />sampling biases. <br />10 <br />