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Twentymile Coal Company - Site 1 SP Chronic Whole Effluent Te; ic-ity Results <br />Oj ,' I f(?- C rl <br />Summary of Laboratory Conditons & Bioassay Results <br />Parameter Ceriodaphnia duhia Pimephales promelas <br />Test Type 7-day static renewal 7-day static renewal <br />Temperature (deg C) 25 +/ - 1 deg C 25 +/ - 1 deg C <br />Light Quality ambient lab levels ambient lab levels <br />Light Intensity (footcandles) 50-100 ft-c 50-100 ft-c <br />Photoperiod 16 hr light/ 8 hr dark 16 hr light/ 8 hr dark <br />Test Chamber Size 40 mL 500 mL <br />Test Solution Volume 30 mL 300 mL <br />Renewal of Test Solutions every 24 hours every 24 hours <br />Age of Test Organisms less than 24 hours less than 24 hours <br /># of neonates per test chamber 1 10 <br /># replicate test chambers per conc. 10 4 <br />Feeding regime 0.2 mL algae/YTC per day 0.15 mL A. nauplli 2xdaily <br />Dilution Water MHFSW MHFSW <br />Test Duration 7-8 days 7-days <br />Effluent Sampling Events and MHFSW Inorganic Chemistries <br />Parameter Sampling Event 1 Sampling Event!- ! Sampling Event 3 MHFSW <br />pH 7.59 7.52 7.40 8.00 <br />Dissolved Oxygen (mg/L) 6.25 5.80 5.65 6.58 <br />Conductivity (pmhos/cm) 2260 1703 2330 330 <br />Ammonia (mg/L) ND ND ND ND <br />Alkalinity (mg/L) 452.45 383.96 485.45 55.79 <br />Hardness (mg/L) 801.20 675.01 833.25 93.74 <br />Residual Chlorine (mg/L) ND ND ND ND <br />Ceriodaphnia duhia & Pimepha/es promelas Chronic Statistical Endpoints <br />Species Parameter IC25 NOEC LOEC <br />Ceriodaphnia duhia Survival >100% 100% >100% <br />Ceriodaphnia dubia Reproduction >100% 100% >100% <br />Pimephales promelas Survival >100% 100% >100% <br />Pimephalespromelas Growth >100% 100% f-->100% <br />Ceriodaphnia dubia Survival and Reproduction Data <br />Concentration Number Exposed Percent Survival Mean Average Number of Neonates <br />Control 10 100% 35.1 <br />12.5% 10 100% 33.8 <br />25% 10 100% 32.4 <br />50% 10 100% 33.1 <br />75% 10 100% 32.4 <br />100% 10 100% 34.8 <br />P