2009-04-30_HYDROLOGY - C1982056 (5)

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Aqua-Tox, Inc. 4880 Robb Street, Suite 8 Wheat Ridge, CO 80033 J03) 621-4880 aqua-tox.com `- ACUTE TOXICITY REPORT Pimephales promelas - Fathead Minnow CLIENT NAME: Twentymile Coal Company SAMPLE I.D.: 033109-1 50 % MORTALITY TEST: Pass TEST RESULT LC50: >100% % SURVIVAL IN 100% DILUTION 70% SAMPLE DATE: 3-30-09 TIME: 13:35PM CLIENT ID: Site 62 ORGANISM: Fathead Minnow AGE: DOH 3-27-09 TEST START: 3-31-09 11-40AM TEST FINISH: 4-4-09 1140AM TYPE OF TEST LC50, 96 hours, static renewal moderately hard fresh synthetic dilution water. MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT AMMONIA. ND D.O 7.40 mg/L HARDNESS: 98.15 mg/L CHLORINE ND ALKALINITY 55.56 mg/L TEMPERATURE 20.0 deg C pH. 7.90 SURVIVAL DATA DILUTIONS (%) DATE (HOURS) CONTROL 6.25 12.5 25 r) n 7.r, inn 3/31/2009 INITIAL 20 20 20 20 20 20 20 4/1/2009 24 20 20 20 20 20 20 20 4/2/2009 48 20 20 20 20 20 20 20 4/3/2009 72 20 20 20 20 20 20 14 4/4/2009 96 20 20 20 20 20 19 14 MORTALITY: 0 0 0 0 0 1 6 SURVIVAL 100`/, 100°G 100`::4 100°,0 100% 95°.4 70`%% DAILY CHEMISTRY - (MfN/MAX) DILUTIONS (%) CONTROL 6.25 12.5 25 50 75 100 CONDUCTIVITY (micros) 314/327 558/567 770/793 1157/1201 1871/1934 2830/2880 3530/3590 D.O. (mg/L) 50/7.4 5.0/7.9 5.0/8.0 5.1/8.0 5 0/8.1 49/8,2 47/8.3 pH 72/7.9 7.4/80 7.6/8.1 7.8/8.2 8.0/8.3 7.9/8.2 7.9/8.0 TEMPERATURE (deg C) 19.7/20.3 19.7/20.3 19.7/20.3 197/20.3 197/20.3 19.7/20.3 19.7/203 GENERAL CHEMISTRY - SAMPLE AMMONIA: 0.04 mg/L D.O.: 8.29 mg/L HARDNESS: 1962 94 mg/L CHLORINE' ND ALKALINITY 422.93 mg/L TEMPERATURE: 20.0 deg C pH 7.86 D.O AFTER AERATION: NA ANOEC CALCULATION METHOD METHOD REFERENCE 75% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg 78 ADDITIONAL COMMENTS Analysis Conducted by: i /?X" Reviewed by: Date: Date: rr'ti qC' vil