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Table 1 - Colorado Acute Test Conditions <br />Parameter C. dubia Fathead Minnow <br />Test Type Static Renewal Static Renewal <br />Temperature 20 +/- 1 ° C 20 +/- 1 ° C <br />Photoperiod 16 hr light/8 hr dark 16 hr light/8 hr dark <br />Test Chamber Size 30 ml 600 ml <br />Test Sample Volume 20 ml 300 ml <br />Renewal Period 24 hrs 24 hrs <br />Age of Organisms < 24 hrs 1 to 14 days <br />Number per Chamber 4 10 <br />Chambers per Concentration 5 4 <br />Feeding Regime per Chamber none 0.1 ml Brine Shrimp @ 48 hours <br />Test Duration 48 hrs 96 hrs <br />Table 2 - Methods and Equipment <br />Characteristic Method/Equipment Method <br />Alkalinity Titrimetric SM 2320 <br />Ammonia CHEMetrics Colorimetery K-1510 <br />Chlorine, Total Residual CHEMetrics Colorimetery K-2504 <br />Conductivity Orion Model 160 Conductivity Meter SM 2510 <br />Dissolved Oxygen YSI Model 59 DO Meter EPA 360.1 <br />Hardness Titrimetric SM 2340 B & EPA 200.7 <br />pH Titrimetric SM 4500 H <br />Temperature NIST5 calibrated thermometer <br />Analytical Balance Mettler AE 240 <br />References <br />Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental <br />Monitoring Systems Laboratory - Cincinnati (EMSL-CI), EPA-600/4-79-020, Revised March 1983. <br />Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality <br />Control Division, Denver, Colorado 80246-1530. <br />US EPA,. October 2002, Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to <br />Freshwater and Marine Organisms, Fifth Edition, U.S. Environmental Protection Agency, Office of Water, <br />Washington, D.C. EPA-821-R-02-012. <br />6 National Institute of Standards and Technology