2009-04-15_HYDROLOGY - C1981008

• Permit No: C1981008
• IBM Index Class Name: HYDROLOGY
• Doc Date: 4/15/2009
• Doc Name: March 2009 DMRS ‎(CO-0000213)‎
• From: Western Fuels Colorado
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: MLT
• Media Type: D
• Archive: No

Table of Contents
Abstract
Methods
Results
Narrative & Discussion
Table 1 - Colo. Test Conditions
Abstract
Table 2 - Methods and Equipment
References
Discharge Monitoring Report Form
Reference Toxicity Data Chart(s)
Supplement Forms'
SGS North America Inc. conducted a two species acute bioassay on Western Fuels New Horizon Mine
effluent discharge. This discharge has been designated as outfall 011. The purpose of this bioassay
was to determine if the facility complies with the acute WET portion of the Colorado State Industrial
General Minimal Discharge Permit. The test organisms utilized were the fathead minnow (Pimephales
promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was not
acutely toxic to fathead minnow or Ceriodaphnia dubia survival. Therefore, outfall 011 is in
compliance with the acute WET portion of its Colorado State Discharge Permit for the first
quarter of 2009.
Methods
A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine,
outfall 011. An effluent grab sample was collected on March 16, 2009, stored on ice, and delivered to
SGS North America Inc. on the next day. The effluent sample was received at a temperature of 3.0°C
and assigned sample tracking number 411-090-BIOA-009. The bioassay was initiated approximately 24
hours after sample collection.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water. Control replicates were exposed to 100% laboratory water.
Statistically, the Binomial method determined the LC50's2. Significant differences in mortality were
analyzed using the Wilcoxon Rank Sum Test and Fisher's Exact test.
' Supplemental forms include statistical analysis and chain-of-custody forms
2 LC5o - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test
2