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• Statistical Methods <br />STATISTICAL DESIGN OF SAMPLING <br />Cover and herbaceous production data were collected in all areas. Woody plant density samples <br />were collected in the 2004 and 2006 Reclaimed Areas and in the Shrub Concentration and <br />Random Sampling Areas as described above, but woody plant density samples were not <br />collected in any of the reference areas or the 2007reclaimed area. Sampling proceeded using a <br />single stage statistical design. <br />Sample locations in reclaimed areas were randomly plotted by use of an internal script routine <br />within ArcGIS 9.2 software. Minimum spacing between sample points was 170 feet (one 50 m <br />transect length). <br />In the extended reference areas, each reference unit was gridded and numbered. Grid cell size <br />was 200 feet X 200 feet. These grid cells were placed contiguously by hand on maps of 1:4,800 <br />scale. Sample locations were selected as the center points of grid cells selected using random <br />numbers generated by computer. <br />Sample points were located in the field using Garmin III handheld GPS units and topographic <br />• maps. Cover and woody plant density transects were located and oriented using, at a glance, the <br />seconds reading on a wristwatch x 6 as an azimuth or a blind throw of a meter stick . The final <br />location of each herbaceous production plot was chosen with a blind throw of the sampling hoop <br />made from the origin of the associated cover/woody plant density transect. At each cover/woody <br />plant density sampling location, the slope (percent) and aspect of the site were determined and <br />recorded, as was the orientation of the transect (in degrees not corrected for magnetic <br />declination). <br />STATISTICAL METHODS <br />Sample adequacy calculations for parameter estimation were carried out using the formula as <br />prescribed by the Colorado Mined Land Reclamation Board for parameter estimation without <br />hypothesis testing (DMG 1995): <br />t 2 s2 <br />Nmin= _ <br />(d X )2 <br />where: <br />• <br />t« = one-tailed t-value with (n-1) degrees of freedom, a=0.10 <br />4