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I. Irrigation treatment - For this experiment rainfall was plentiful and not typical for the first two <br />years during the study and soil moisture was relatively high even in un-irrigated plots, as <br />indicated by soil moisture matrix potential values and low leaf water potential data for all <br />treatments. This prevented a good examination of the irrigation treatment effects. Aspen growth <br />and survival did not appear to be dependent on, or in some cases consistent with, irrigation <br />treatment, suggesting that soil moisture from the frequent rain events was sufficient even in the <br />non-irrigated plots. The supposition of adequate moisture available to all trees is further evident <br />in that there appeared to be no relationship between irrigation treatment and average leaf area, <br />total leader growth, terminal leader growth, stem diameter growth or caliper, or survival (data <br />previously shown in earlier reports). Growth of second, third, and fourth lateral branches <br />appeared to be similar for all treatments, but are reflected in total growth. Pre-dawn leaf water <br />potential levels also indicate moisture stress was generally less than 8 bars (0.8 mPa) pressure, <br />and did not appear to be related to irrigation treatments during the years when these <br />measurements were taken. <br />II. Transplant type -The aspen saplings used in the irrigation study that were transplanted from <br />the Yoast site exhibited considerably more injury and had considerably more disease infections <br />than natural sprouts arising from buried root segments or potted plant. Transplant shock was <br />evident only the first year. Leaf area growth, leader growth, stem diameter growth, and survival <br />were considerably less with these plants than with natural sprouts or potted plants during the first <br />year <br />of the study, but the transplanted trees grew well the second and third year of the study <br />(depending on treatment). Potted plants survival was 100% and growth on these trees appeared <br />better than transplanted cuttings the first year. Growth of the transplants was better than potted <br />plants in subsequent years. <br />III. Soil type -Roto-cleared soil provided sufficient natural sprouting to provide an adequate <br />stand of aspen trees, and these trees appeared to grow better and survival appeared higher than <br />adjacent transplanted trees growing in the same soil in the first two years of the study. Dozer- <br />cleared soil which had been temporarily stored, had considerably lower numbers of natural <br />sprouts than roto-cleared soil, and stocking was sparse (data not shown). Natural sprouts <br />appeared to have greater total leaf area and greater stem diameter growth on roto-cleared soil <br />than dozer-cleared soil, but terminal leader growth appeared similar on both soil types (data <br />presented in earlier reports). Natural root sprouts had no lateral branches. Leaves also appeared <br />to be larger on these trees (data not shown). Nevertheless, these trees apparently experienced <br />somewhat greater pre-dawn water stress in July and September than trees in the irrigated <br />treatments, including the irrigated controls with no water added. The data suggest that pre-dawn <br />water stress levels as high as 14 bars, and afternoon water stress levels as high as 20-25 bars, <br />were not of sufficiently high levels to cause enough stress to reduce survival or growth of these <br />trees. Soil moisture stress appeared to be less with transplanted sprouts in the irrigation <br />experiment, including the un-irrigated controls, than with natural root sprouts or potted plants. It <br />is interesting to note that leaves appeared smaller and terminal growth appeared less on these <br />apparent less-stressed transplanted trees, suggesting that growth of root sprouts, potted plants, <br />and natural sprouts was not limited by the apparent higher moisture stress levels they <br />experienced. Maximum leaf water potentials at mid-afternoon found stress levels of about 25 <br />bars or less, levels that appeared unrelated to treatment, or to growth and survival.