2009-02-02_HYDROLOGY - C1982056 ‎(5)‎

• Permit No: C1982056
• IBM Index Class Name: HYDROLOGY
• Doc Date: 2/2/2009
• Doc Name: 4th Quarter 2008 DMRS ‎(CO-0034142)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JHB
• Media Type: D
• Archive: No

Aqua-Tox, Inc.
4880 Robb Street, Suite -
fM Wheat Ridge, CO 80033
! [phone] aqua-tox.lriYm;
-h?
w? ACUTE TOXICITY REPORT
r Cerioda hnia dubia
CLIENT NAME: Twentymile Coal Company SAMPLE I.D.: 121908-1
50 % MORTALITY TEST: Pass
% SURVIVAL IN 100% DILUTION: 100% TEST RESULT LC50: >100%
SAMPLE DATE: 12-18-08 TIME: 10:45AM CLIENT ID: Site 84
ORGANISM: Ceriodaphnia dubia AGE: < 24 hours old
TEST START: 12-19-08 18:OOPM TEST FINISH: 12-21-08 18:OOPM
TYPE OF TEST: LC50, 48 hours, static renewal moderately hard fresh synthetic dilution water.

MODERATELY HARD FRESH SYNTHETIC DILUTION WATER CONSTITUENT
AMMONIA: ND D.O : 7 30 mg/L
HARDNESS: 91.50 mg/L CHLORINE: ND
ALKALINITY: 55.96 mg/L TEMPERATURE: 19.7 deg C
pH: 8.10
SURVIVAL DATA DILUTIONS (%)
DATE (HOURS)CONTROL 6.25 125 25 50 75 100
12/19/2008 INITIAL 20 20 20 20 20 20 20
12/20/2008 24 20 20 20 20 20 20 20
12/21/2008 48 20 20 20 20 20 20 20
MORTALITY 0 0 0 0 0 0 0
% SURVIVAL- 100% 100% 100% 100% 100% 100% 100%

DAILY CHEMISTRY - (MIN/MAX)
0 6.25
12.5 DILUTIONS (%)
25
50
75 100
CONDUCTIVITY (micros) 316/332 615/642 936/960 1498/1532 2930/3000 4110/4130 5190/5300
D.O (mg/L) 7.3/7.6 7.5/7.6 7.5/7.7 7.6/7.7 7.5/7.8 7.6/8.1 7.6/8.4
pH 8.1/8.2 8.2/8.3 8.2/8.4 83/8.5 8.2/8.7 8.2/8.7 8.2/8.5
TEMPERATURE (deg C) 19.1/20 2 19.1/20.2 19.1/20.2 19,1/20.2 19.1/20.2 19.1/20.2 19.1/20.2
L-
GENERAL CHEMISTRY - SAMPLE
AMMONIA. 1 12 mg/L D.O.: 8.36 mg/L
HARDNESS: 1000 mg/L CHLORINE: ND
ALKALINITY 484 69 mg/L TEMPERATURE: 19.7 deg C
pH 8.20 D.O AFTER AERATION: NA
ANOEC CALCULATION METHOD METHOD REFERENCE
100% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg.78
ADDITIONAL COMMENTS:
Analysis
Conducted by: A/Z ,=-z,.oe Reviewed by:
Date: Date: ! a z