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PART I <br />Page No. 5 <br />Petmit No. CO-0044377 <br />• A. DEFINITION OF EFFLUENT LIATITATIONS <br />2. Acute FVET Tesline-Outfttll 001 (continued) <br />(b) Failure of Test and Division Notification <br />An acute 1VL-T test is failed whenever the LCD, which represents an estimate o(the effluent concentration which is <br />lethal to 50 0 0( the test organisms in the time period prescribed by the test, is found to be less than or equal to <br />100% ef0ucnt. The pcrmittec must provide ss•riitcn notification of the failure of a WET test to the Division, along <br />with a statement as to svltcthcr the Preliminary Toxicity Incident ("PTI")/Toxicity Identification Evaluation ('TIE') <br />investigation or accelerated testing is being performed (see next section). Notification must be received by the <br />Division within 14 calendar days of the dcntonstralion of acute WET in the routine required test. <br />'Demonstration' for the purposes of Pans I.A.2.(b),(c),(d) and (t) means no later than the last day of the <br />laboratory test. <br />(c) Automatic Compliance Schedule Utwn Failure of Test <br />IC a routine acute 1VET test is failed, the following automatic compliance schedule shall apply. As part of this the <br />permittee shall either: <br />(i) proceed to conduct the PTI/TIE im•estigation as described in Pan I.A.2.(d), or <br />(ii) conduct accelerated testing using the single species found to be more sensitive. <br />• If accelerated testing is being performed, the pcrmittec shall provide arittcn notification oC the resuhs within <br />14 calendar da}•s of contplelion of the "Pattern of Toxicity"/"No Toxicity" demonstration. Testing will be at <br />least once every two weeks for up to five tests until I) two consecutive tests fail or three of five tests fail, in which <br />case a pattern of toxicity has been demonstrated or, 2) two consecutive tests pass or three of five tests pass, in <br />which case no pattem of toxicity has been found. IF no pattern of toxicity is found the toxicity episode is considered <br />to be ended and routine testing is to resume. [f a pattem of toxicity is found, a PTl/TIE investigation is to be <br />performed. If a pattern oftoxicity is not demonstrated but a significant level of erratic toxicity is found, the <br />Division may require an increased frequency of routine monitoring or some other modified approach. <br />(d) PTI/TIE <br />The results of the PTI/TIE investigation are to be received by the Division within 120 days of the <br />demonstration of acute WET in the routine test, as defined above, or if accelerated testin is performed, the <br />date the pattern of toxicity is demonstrated. A status report is to be provided to the Division at the 30. 60 <br />and 90 day Doints of the PTI/TIE investieation. The Division may ez[end the time frame for investigation where <br />reasonable justification exists. A request for an extension must be made in writing and received prior to the 120 day <br />deadline. Such request must include a justification and supporting data for such an extension. <br />The permittee may use the time for investigation to conduct a PTI or move directly into the TIE. A PTI consists of <br />a brief search for possible sources oC WET, which might reveal causes of such toxicity and appropriate corrective <br />actions more simply and cos[ effectively than a Cotmal TIE. If the PTI allows resolution of the WET incident, the <br />TIE need not necessarily be conducted. If, however, WET is not identified or resolved during the PTI, the TIE <br />must be conducted within the allowed 120 day time frame. <br />Any permittee that is required to conduct a PTl/TIE investigation shall do so in conformance wi[h procedures <br />identified in the following documents, or as subsequently updated: 1) Methods far Aquatic Toxicity Identification <br />• Evaluations. Phase 1 Toxicity Characterivyion'Procedures, EPA/600/6-91/003 Feb. 91 and 2) Methods for Aouatic <br />Toxicity Identification Evaluations Phase 11 Toxicity Identification Procedures, EPA/600/3-88/035 Feb. 1989. <br />ixnar nw..n.. u, row <br />