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September 1996 -9- 963-2128.002 <br /> Fresquez et al. (1991), found that in blue grams grown on biosolid-amended soil, that Cu <br /> and Cd tissue concentrations in the treated plots did not exceed recommended levels at any <br /> time during the study and were not significantly different from those in the control plots after <br /> the fifth growing season. <br /> To determine the plant tissue Cu concentrations in species growing on disturbed and <br /> undisturbed land, Climax collected plant tissue samples for analysis. Tissue samples were <br /> collected from the two different grass species growing on four separate locations. Plants <br /> were sampled by collecting the entire live, above-ground biomass. Plants were not washed <br /> prior to digestion, therefore decontamination of air-borne particles was not ensured. The <br /> locations and grass species sampled are as follows: <br /> ► One fescue (Festuca spp.) grown on native, undisturbed area (NU) <br /> ' One timothy (Phleum spp.) grown on a biosolids-amended, waste rock <br /> (AV) <br /> ► One fescue and one timothy grown on one disturbed site not amended with <br /> biosolids (D) <br /> Plant tissue samples were analyzed by microwave nitric acid digestion (AOAC) and the <br /> digested solutions were analyzed by inductively coupled argon plasma - flame emission <br /> spectroscopy (USEPA 1986b). The results from this sampling program are presented in <br /> Table 2. <br /> TABLE 2 <br /> CU CONCENTRATIONS (PPM ON A DRY WEIGHT BASIS) IN PLANTS <br /> GROWING ON BIOSOLIDS-AMENDED AND UNAMENDED SOIL <br /> .:.............:..............:.......... ::::::.g.E..].i:Iy::;�::�:y::..:::::::::::.: <br /> %:y7t51PtxAwC� <br /> .....:{......:i::::.:.�::.:vw::x.}w:;. •a.4; {. :,:.:-::.. � �+t}%i::::: :i::';i?i:;:;%i' <br /> Av... <br /> }x} ::::•: <br /> %::;i^:i;:}r'+::w:{:.}Y::::•}':::::.}jvr J.:�::rv:•}::{}}:vj•}S:}}:4:is <br /> :: ....... - ..�:•}:ham: <br /> Cu Conc. 8 3 8 5 <br /> (ppm) n=1 n=1 n=1 n=1 <br /> r:URvm21=129r,MM Golder associates <br />