2008-10-30_HYDROLOGY - C1982056 ‎(5)‎

• Permit No: C1982056
• IBM Index Class Name: HYDROLOGY
• Doc Date: 10/30/2008
• Doc Name: 3rd Quarter 2008 DMRS ‎(CO-0042161)‎
• From: Twentymile Coal Company
• To: WQCD
• Permit Index Doc Type: DMR’s
• Email Name: JHB
• Media Type: D
• Archive: No

Aqua-Tox, Inc.
4880 Robb Street, Suite 8
Wheat Ridge, CO 80033
[phone] aqua-tox.com
ACUTE TOXICITY REPORT
)hales promelas - Fathead Minnow
LIENT NAME: Twentymile Coal Company SAMPLE I.D.: 090908-2
% MORTALITY TEST: Pass TEST RESULT LC50: >100%
SURVIVAL IN 100% DILUTION: 100%
4MPLE DATE:-9-8-08 TIME: 12:57PM CLIENT ID: Site 115
RGANISM: Fathead Minnow AGE: DOH 9-5-08
.ST START: 9-9-08 12:31 PM TEST FINISH: 9-13-08 12:31 PM
(PE OF TEST: LC50, 96 hours, static, renewal, moderately hard fresh synthetic dilution water.
ODERATEL
AMMONIA: ND
HARDNESS: 90 mg/L
ALKALINITY: 54.96 mg/L
pH: 8.00
TION WATER CONSTITUENT
D.O.: 7.20 mg/L
CHLORINE: ND
TEMPERATURE: 19.7 deg C
DATE HOURS CONTROL 6.25
9/9/2008 INITIAL
9/10/2008 24
9/11/2008 48
9/12/2008 72
9/13/2008 96
MORTALITY:
% SURVIVAL:
DILUTIONS (%)
125 25 sn 7-S inn
20 20 20 20 20 20 20
20 20 20 20 20 20 20
20 20 20 20 20 20 20
20 20 19 20 20 20 20
20 20 19 20 20 20 20
0 0 1 0 0 0 0
100% 100% 95% 100% 100% 100% 100%
41LY CHEMISTRY - (MIN/MAX) DILUTIONS (%)
CONTROL 6.25 12.5 25 50 75 100
CONDUCTIVITY (micros)
D.O. (mg/L)
pH
TEMPERATURE (deg C)
340/352 690/710 1026/1039 1677/1746 3070/3140 4280/4330 5440/5560
4.9/7.2 4.9/7.4 5.3/7.5 5.1/7.9 5.4/8.5 5.0/9.1 5.6/9.8
7.6/8.2 7.6/8.2 7.7/8.3 8.0/8.5 8.2/8.5 8.2/8.4 8.1/8.4
19.5/20.2 19.5/20.2 19.5/20.2 19.5/20.2 19.5/20.2 19.5/20.2 19.5/20.2
GENERAL CHEMISTRY - SAMPLE
AMMONIA: 2.05 mg/L D.O.: 9.80 mg/L
HARDNESS: 428 mg/L CHLORINE: ND
ALKALINITY: 740.53 mg/L TEMPERATURE: 19.7 deg C
pH: 8.12 D.O AFTER AERATION: NA
'ANOEC; -CALCULATION METHOD 'METHOD 'REFERENCE
' 100% SPEARMAN-KARBER EPA -821-R-02-012 October 2002 pg.78
ADDITIONAL COMMENTS:
Analysis
Conducted by: Reviewed by:
Date: Date: