2008-10-24_HYDROLOGY - C1981008

• Permit No: C1981008
• IBM Index Class Name: HYDROLOGY
• Doc Date: 10/24/2008
• Doc Name: Wet Test Discharge for Outfalls 007 and 011
• From: Western Fuels-Colorado
• To: WQCD
• Permit Index Doc Type: NPDES
• Email Name: MLT
• Media Type: D
• Archive: No

Table of Contents
Abstract
Methods
Results
Narrative & Discussion
Table 1 - Colorado Test Conditions
Abstract
Table 2 - Methods and Equipment
References
Discharge Monitoring Report Form(s)
Reference Toxicity Data Chart(s)
Supplement Forms'
SGS North America, Inc. conducted a two species chronic bioassay on Western Fuels New Horizon Mine
effluent discharge. This discharge has been designated as outfall 007. The purpose of this bioassay
was to determine if the facility complies with the chronic WET portion of the Colorado State Permit
System Authorization to Discharge. The test organisms utilized were the fathead minnow (Pimephales
promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was not
chronically toxic to fathead minnow or to Ceriodaphnia dubia survival. Therefore, outfall 007 is in
compliance with the chronic WET portion of its Colorado State Discharge Permit for the third
quarter of 2008.
Methods
A two species chronic bioassay was conducted with effluent from Western Fuels New Horizon Mine,
Outfall 007. An effluent composite sample was collected on September 15, 2008, stored on ice, and
delivered to SGS North America Inc. the next day. The effluent samples were received at a temperature
of 10.5° C and assigned sample tracking number 411-080-BIOA-069. The client was contacted via
telephone regarding the receiving temperature and gave verbal okay to proceed with test initiation. The
bioassay was initiated approximately 24 hours after sample collection.
Subsequent samples were collected on September 17, 2006, stored on ice, and delivered to SGS North
America Inc. the following day. The effluent samples were received at a temperature of 6.0°C. Because
of the remote location of the facility, two concurrent samplings events were utilized for this examination.
Sufficient sample volume was collected for the entire test.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water. Control replicates were exposed to 100% laboratory water.
Statistically, fathead minnow survival and growth data were analyzed for significant differences using
Steel's Many One Rank Test and Dunnett's test, respectively. Ceriodaphnia dubia survival and
reproduction data were analyzed for significant differences using Fisher's Exact Test and Dunnett's Test,
respectively. IC252 values were determined using the Bootstrap Linear Interpolation method.
1 Supplemental forms include statistical analysis and chain-of-custody forms
2 Inhibition Concentration at 25% reduction