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Narrative and Discussion <br />• PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample: <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature: <br />• TEST ORGANISMS <br />CO-0000213 <br />Effluent Grab <br />09/29/08 09:50 <br />09/30/08 11:00 <br />Reconstituted Laboratory Water <br />1.0°C <br />Pimephales promelas <br />• PROCEDURE <br />Age: 8 days old <br />Acute, 96-hour bioassays as outlined in the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels New Horizon Mine discharge permit (CO-0000213), an acute <br />WET test is failed whenever the LC50, which represents an estimate of the effluent concentration <br />which is lethal to 50% of the test organisms in the time period prescribed by the test, is found to <br />be less than or equal to 100% effluent. <br />To determine if the facility complies with the "Automatic Compliance Schedule" of the <br />acute WET portion of the Colorado State Permit System Authorization to Discharge, SGS <br />North America Inc. conducted an accelerated one species acute bioassay on the Western Fuels <br />New Horizon Mine, outfall 011 discharge. The test organism utilized was Pimephales promelas. <br />The results for the first sample of the accelerated testing schedule indicate this effluent <br />sample was not acutely toxic to P. promelas survival. <br />• DATE OF REPORT <br />October 7, 2008 <br />Report prepared by: Stephan <br />1?? ?-, 6)),?11 ?, <br />Supervisor <br />reen Hawkins <br />Date