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PART I <br />Page No. ! <br />Permit No. COG-50000( <br />testing results for the first calendar quarter ending March 31 shall be reported with the DMR due April 28.) The results shall <br />be submitted on the Chronic Toxicity Test report form, available from the Division. Copies of these reports are to be <br />submitted to the Division along with the DMR. <br />The permittee shall conduct each chronic WET test in general accordance with methods described in Short Term Methods <br />for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600/4-89/001 or the <br />most current edition, except as modified by the most current Division guidance document entitled Guidelines for Conducting <br />Whole Effluent Toxicity Tests. The permittee shall conduct such tests using Ceriodaphnia dubia and fathead minnows. <br />2. Failure of Test and Division Notification <br />A chronic WET test is failed whenever 1) there is a statistically significant difference in lethality between the control and <br />any effluent concentration less than or equal to the instream waste concentration (IWC) and, 2) the IC2S, which represents an <br />estimate of the effluent concentration at which 25% of the test organisms demonstrate inhibition as reflected by lethality, is <br />at any effluent concentration less than or equal to the IWC. The permittee must provide written notification of the failure of. <br />WET test to the Division, along with a statement as to whether a Preliminary Toxicity Investigation (PTI)/Toxicity <br />Identification Evaluation (TIE) or accelerated testing is being performed (see Part I.B.7.c.). Notification must be received <br />by the Division within 21 calendar days of the demonstration of chronic WET in the routine required test. <br />Demonstration means no later than the last day of the laboratory test. <br />c. Other WET Testing Requirements Applicable to both Chronic and Acute WET Testing <br />1. Automatic Compliance Schedule Upon Failure of Test <br />If a routine chronic WET test is failed, regardless of whether the limit is in effect, the following automatic compliance <br />schedule shall apply. As part of this, the permittee shall either: <br />i. Proceed to conduct the PTI/TIE investigation as described in Part I.B.7.c., or <br />ii. Conduct accelerated testing using the single species found to be more sensitive. <br />If accelerated testing is being performed, the permittee shall provide written notification of the results within 14 calendar <br />days of completion of the Pattern of Toxicity/No Toxicity demonstration. Testing will be at least once every two weeks for <br />up to five tests until; 1) two consecutive tests fail or three of five tests fail, in which case a pattern of toxicity has been <br />demonstrated or 2) two consecutive tests pass or three of five tests pass, in which case no pattern of toxicity has been found. <br />If no pattern of toxicity is found the toxicity episode is considered to be ended and routine testing is to resume. If a pattern <br />of toxicity is found, a PTI/TIE investigation is to be performed. If a pattern of toxicity is not demonstrated but a significant <br />level of erratic toxicity is found, the Division may require an increased frequency of routine monitoring or some other <br />modified approach. <br />2. PTI/TIE <br />The results of the PTI/TIE investigation are to be received by the Division within 120 days of the demonstration of chronic <br />WET in the routine test, as defined above, or if accelerated testing is performed, the date the pattern of toxicity is <br />demonstrated. A status report is to be provided to the Division at the 30, 60 and 90 day points of the PTI/TIE investigation. <br />The Division may extend the time flame for investigation where reasonable justification exists. A request for an extension <br />must be made in writing and received prior to the 120 day deadline. Such request must include a justification and supportin <br />data for such an extension. <br />The permittee may use the time for investigation to conduct a PTI or move directly into the TIE. A PTI consists of a brief <br />search for possible sources of WET, which might reveal causes of such toxicity and appropriate corrective actions more <br />simply and cost effectively than a formal TIE. If the PTI allows resolution of the WET incident, the TIE need not <br />necessarily be conducted. If, however, WET is not identified or resolved during the PTI, the TIE must be conducted within <br />the allowed 120 day time frame. <br />Any permittee that is required to conduct a PTI/TIE investigation shall do so in conformance with procedures identified in <br />the following documents, or as subsequently updated: 1) Toxicity Identification Evaluation: Characterization of Chronicall <br />Toxic Effluents, Phase I, EPA/600/6-91/005F May 92, 2) Methods for Aquatic Toxicity Identification Evaluations, Phase I <br />Toxicity Characterization Procedures, EPA/600/6-91/003 Feb. 91 and 3) Methods for Aquatic Toxicity Identification <br />Evaluations, Phase II Toxicity Identification Procedures, EPA/600/3-88/035 Feb. 1989. <br />A fourth document in this series is Methods for Aquatic Toxicity Identification Evaluations, Phase III Toxicity Confirmatic <br />Procedures, EPA/600/3-88/036 Feb. 1989. As indicated by the title, this procedure is intended to confirm that the suspectei